Structural characterisation of macromolecular complexes

Application deadline: 10 March 2024. Course participants will receive a comprehensive overview of state-of-the-art structural methods, with a focus on sample preparation, characterisation, and data integration strategies. Key aspects covered by lectures and practicals include multi-subunit protein expression in bacteria, insect, and mammalian cells; isolation of native complexes and in-vitro reconstitution of protein and protein-nucleic acid complexes; biochemical and biophysical characterization to assess stoichiometry and sample homogeneity; 3D structure determination of macromolecular assemblies at different resolution regimes; and the integration of data from multiple experimental sources (X-ray crystallography, cryo-EM, SAXS, NMR, mass spectrometry, smFRET, and super-resolution light microscopy).