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Recent research news on Artificial Biology

27 November 2023 - Research news

In this work, synthetic cells equipped with an artificial signaling pathway that connects an extracellular trigger event to the activation of intracellular transcription are engineered. Learning from nature, this is done via an engineering of responsive enzymes, such that activation of enzymatic activity can be triggered by an external biochemical stimulus. Reversibly deactivated creatine kinase to achieve triggered production of adenosine triphosphate, and a reversibly deactivated nucleic acid…

01 May 2023 - Research news

Nanopores are label-free single-molecule analytical tools that show great potential for stochastic sensing of proteins. Here, we described a ClyA nanopore functionalized with different nanobodies through a 5–6 nm DNA linker at its periphery. Ty1, 2Rs15d, 2Rb17c, and nb22 nanobodies were employed to specifically recognize the large protein SARS-CoV-2 Spike, a medium-sized HER2 receptor, and the small protein murine urokinase-type plasminogen activator (muPA), respectively. The pores modified with…

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Ghodke, S. D., Jensen, G. V., Svane, A. S. P., Weise, K., Søndergaard, A., Behrens, M. A., Pedersen, J. S., Nielsen, N. C., Pedersen, J. S., Winter, R. & Otzen, D. (2014). Polymorphism, metastable species and interconversion: the many states of glucagon fibrils. In V. Uversky & Y. Lyubchenko (Eds.), Bio-nanoimaging: Protein misfolding and aggregation (pp. 373-386). Elsevier. https://doi.org/10.1016/B978-0-12-394431-3.00034-1

Zhang, Y., Mian Teo, B., Goldie, K. & Stadler, B. M. (2014). Poly(N-isopropylacrylamide)/Poly(dopamine) Capsules. Langmuir, 30(19), 5592-5598. https://doi.org/10.1021/la5005227

Christiansen, M. C., Hirsch, C. & Schmidt, V. (2014). Prediction of regionalized car insurance risks based on control variates. Statistics Risk Modeling, 31(2), 163-181.

Kjems, J., Ferapontova, E. & Gothelf, K. V. (2014). Preface. In J. Kjems, E. Ferapontova & K. V. Gothelf (Eds.), Nucleic Acid Nanotechnology (pp. ix-x). Springer.

Zhong, X., Yu, H., Zhuang, G., Li, Q., Wang, X., Zhu, Y., Liu, L., Li, X., Dong, M. & Wang, J.-G. (2014). Pyridyne cycloaddition of graphene: External active sites for oxygen reduction reaction. Journal of Materials Chemistry A, 2(4), 897-901. https://doi.org/10.1039/c3ta13449k

Okholm, A. H., Nielsen, J. S., Vinther, M., Sørensen, R. S., Schaffert, D. & Kjems, J. (2014). Quantification of cellular uptake of DNA nanostructures by qPCR. Methods, 67(2), 193-197. https://doi.org/10.1016/j.ymeth.2014.01.013

Zhang, S., Aslan, H., Besenbacher, F. & Dong, MD. (2014). Quantitative biomolecular imaging by dynamic nanomechanical mapping. Chemical Society Reviews, 43(21), 7412-7429. https://doi.org/10.1039/c4cs00176a