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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

27 November 2023 - Research news

In this work, synthetic cells equipped with an artificial signaling pathway that connects an extracellular trigger event to the activation of intracellular transcription are engineered. Learning from nature, this is done via an engineering of responsive enzymes, such that activation of enzymatic activity can be triggered by an external biochemical stimulus. Reversibly deactivated creatine kinase to achieve triggered production of adenosine triphosphate, and a reversibly deactivated nucleic acid…

01 May 2023 - Research news

Nanopores are label-free single-molecule analytical tools that show great potential for stochastic sensing of proteins. Here, we described a ClyA nanopore functionalized with different nanobodies through a 5–6 nm DNA linker at its periphery. Ty1, 2Rs15d, 2Rb17c, and nb22 nanobodies were employed to specifically recognize the large protein SARS-CoV-2 Spike, a medium-sized HER2 receptor, and the small protein murine urokinase-type plasminogen activator (muPA), respectively. The pores modified with…

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Weber, M., Schneider, D., Prodöhl, A., Dreher, C., Becker, C., Underhaug, J., Svane, A. S. P., Malmendal, A., Nielsen, N. C. & Otzen, D. (2011). SDS-facilitated in vitro formation of a transmembrane B-type cytochrome is mediated by changes in local pH. Journal of Molecular Biology, 407(4), 594-606. https://doi.org/10.1016/j.jmb.2011.02.005

Jepsen, M. B., Beerens, N., Nechyponuk-Zloy, V., Besenbacher, F., Kjems, J. & Birkedal, V. (2011). Single molecule FRET microscopy studies of tRNA annealing onto the HIV-1 genome. Poster session presented at International Bunsen Discussion Meeting Förster resonance energy transfer in life science, Germany.

Birkedal, V., Dong, M., Golas, M. M., Sander, B., Andersen, E. S., Gothelf, K. V., Besenbacher, F. & Kjems, J. (2011). Single molecule microscopy methods for the study of DNA origami structures. Microscopy Research and Technique, 74(7), 688-698. https://doi.org/10.1002/jemt.20962

Jahn, K., Olsen, E. M., Nielsen, M. M., Tørring, T., Mohammadzadegan, R., Andersen, E. S., Gothelf, K. V. & Kjems, J. (2011). Site-Specific Chemical Labeling of Long RNA Molecules. Bioconjugate Chemistry, 22(1), 95-100. https://doi.org/10.1021/bc100422k

Søbjerg, L. S., Lindhardt, A. T., Skrydstrup, T., Finster, K. & Meyer, R. L. (2011). Size control and catalytic activity of bio-supported palladium nanoparticles. Colloids and Surfaces B: Biointerfaces, 85(2), 373-378. https://doi.org/10.1016/j.colsurfb.2011.03.014

Wang, J., Jensen, U. B., Jensen, G. V., Shipovskov, S., Balakrishnan, V. S., Otzen, D., Pedersen, J. S., Besenbacher, F. & Sutherland, D. S. (2011). Soft Interactions at Nanoparticles Alter Protein Function and Conformation in a Size Dependent Manner. Nano Letters, 11(11), 4985-4991. https://doi.org/10.1021/nl202940k

Barcena, T. G., Finster, K. & Yde, J. C. (2011). Spatial Patterns of Soil Development, Methane Oxidation, and Methanotrophic Diversity along a Receding Glacier Forefield, Southeast Greenland. Arctic, Antarctic, and Alpine Research, 43(2), 178-188. https://doi.org/10.1657/1938-4246-43.2.178

Kragh, H. & Nielsen, K. H. (2011, Dec). Spreading the gospel: The Bohr atom popularised. http://arxiv.org/abs/1112.2499

Hatch, R. C., Bianchi, M., Guan, D., Shining, B., Iversen, B. B., Nilsson, L., Hornekær, L. & Hofmann, P. (2011). Stability of the Bi2Se3(111) topological state: Electron-phonon and electron-defect scattering. Physical Review B, 83, 241303. https://doi.org/10.1103/PhysRevB.83.241303

Zwieb, C., Nakao, Y., Nakashima, T., Takagi, H., Goda, S., Andersen, E. S., Kakuta, Y. & Kimura, M. (2011). Structural modeling of RNase P RNA of the hyperthermophilic archaeon Pyrococcus horikoshii OT3. Biochemical and Biophysical Research Communications, 414(3), 517-522. https://doi.org/10.1016/j.bbrc.2011.09.098

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Kalinka Hansen