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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

27 November 2023 - Research news

In this work, synthetic cells equipped with an artificial signaling pathway that connects an extracellular trigger event to the activation of intracellular transcription are engineered. Learning from nature, this is done via an engineering of responsive enzymes, such that activation of enzymatic activity can be triggered by an external biochemical stimulus. Reversibly deactivated creatine kinase to achieve triggered production of adenosine triphosphate, and a reversibly deactivated nucleic acid…

01 May 2023 - Research news

Nanopores are label-free single-molecule analytical tools that show great potential for stochastic sensing of proteins. Here, we described a ClyA nanopore functionalized with different nanobodies through a 5–6 nm DNA linker at its periphery. Ty1, 2Rs15d, 2Rb17c, and nb22 nanobodies were employed to specifically recognize the large protein SARS-CoV-2 Spike, a medium-sized HER2 receptor, and the small protein murine urokinase-type plasminogen activator (muPA), respectively. The pores modified with…

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Recent publications by network

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Mazur, F., Bally, M., Stadler, B. M. & Chandrawati, R. (2017). Liposomes and lipid bilayers in biosensors. Advances in Colloid and Interface Science, 249, 88-99. https://doi.org/10.1016/j.cis.2017.05.020

Frislev, H. S., Boye, T. L., Nylandsted, J. & Otzen, D. (2017). Liprotides kill cancer cells by disrupting the plasma membrane. Scientific Reports, 7(1), Article 15129. https://doi.org/10.1038/s41598-017-15003-6

Nærdal, I., Netzer, R., Irla, M., Krog, A., Heggeset, T. M. B., Wendisch, V. F. & Brautaset, T. (2017). L-lysine production by Bacillus methanolicus: Genome-based mutational analysis and L-lysine secretion engineering. Journal of Biotechnology, 244, 25-33. https://doi.org/10.1016/j.jbiotec.2017.02.001

Chandrawati, R., Chang, J. Y. H., Reina-Torres, E., Jumeaux, C., Sherwood, J. M., Stamer, W. D., Zelikin, A. N., Overby, D. R. & Stevens, M. M. (2017). Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma. Advanced Materials, 29(16), Article 1604932. https://doi.org/10.1002/adma.201604932

Schandock, F., Riber, C. F., Röcker, A., Müller, J. A., Harms, M., Gajda, P., Zuwala, K., Andersen, A. H. F., Løvschall, K. B., Tolstrup, M., Kreppel, F., Münch, J. & Zelikin, A. N. (2017). Macromolecular Antiviral Agents against Zika, Ebola, SARS, and Other Pathogenic Viruses. Advanced Healthcare Materials, 6(23), Article 1700748. https://doi.org/10.1002/adhm.201700748

Riber, C. F., Hinton, T. M., Gajda, P., Zuwala, K., Tolstrup, M., Stewart, C. & Zelikin, A. N. (2017). Macromolecular Prodrugs of Ribavirin: Structure-Function Correlation as Inhibitors of Influenza Infectivity. Molecular Pharmaceutics, 14(1), 234-241. https://doi.org/10.1021/acs.molpharmaceut.6b00826

Banerjee, C., Westberg, M., Breitenbach, T., Bregnhøj, M. & Ogilby, P. R. (2017). Monitoring Interfacial Lipid Oxidation in Oil-in-Water Emulsions Using Spatially Resolved Optical Techniques. Analytical Chemistry, 89(11), 6239-6247. https://doi.org/10.1021/acs.analchem.7b01228

Taipaleenmäki, E. M., Mouritzen, S., Schattling, P., Zhang, Y. & Stadler, B. M. (2017). Mucopenetrating Micelles with a PEG Corona. Nanoscale, 9(46), 18438-18448. https://doi.org/10.1039/C7NR06821B

Liu, Y., Zhou, G., Liu, Z., Guo, M., Jiang, X., Taskin, M. B., Zhang, Z., Liu, J., Tang, J., Bai, R., Besenbacher, F., Chen, M. & Chen, C. (2017). Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration. Scientific Reports, 7(1), 8197. Article 8197. https://doi.org/10.1038/s41598-017-08572-z

Mortensen, H. G., Madsen, J. K., Andersen, K. K., Vosegaard, T., Deen, G. R., Otzen, D. E. & Pedersen, J. S. (2017). Myoglobin and α-Lactalbumin Form Smaller Complexes with the Biosurfactant Rhamnolipid Than with SDS. Biophysical Journal, 113(12), 2621-2633. https://doi.org/10.1016/j.bpj.2017.10.024

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Kalinka Hansen