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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

27 November 2023 - Research news

In this work, synthetic cells equipped with an artificial signaling pathway that connects an extracellular trigger event to the activation of intracellular transcription are engineered. Learning from nature, this is done via an engineering of responsive enzymes, such that activation of enzymatic activity can be triggered by an external biochemical stimulus. Reversibly deactivated creatine kinase to achieve triggered production of adenosine triphosphate, and a reversibly deactivated nucleic acid…

01 May 2023 - Research news

Nanopores are label-free single-molecule analytical tools that show great potential for stochastic sensing of proteins. Here, we described a ClyA nanopore functionalized with different nanobodies through a 5–6 nm DNA linker at its periphery. Ty1, 2Rs15d, 2Rb17c, and nb22 nanobodies were employed to specifically recognize the large protein SARS-CoV-2 Spike, a medium-sized HER2 receptor, and the small protein murine urokinase-type plasminogen activator (muPA), respectively. The pores modified with…

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Recent publications by network

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Lazar, P., Zhang, S., Šafářová, K., Li, Q., Froning, J. P., Granatier, J., Hobza, P., Zbořil, R., Besenbacher, F., Dong, M. & Otyepka, M. (2013). Quantification of the interaction forces between metals and graphene by quantum chemical calculations and dynamic force measurements under ambient conditions. ACS Nano, 7(2), 1646-1651. https://doi.org/10.1021/nn305608a

Nielsen, S. B. & Otzen, D. (2013). Quartz crystal microbalances as tools for probing protein-membrane interactions. In J. Kleinschmidt (Ed.), Lipid-Protein Interactions: Methods and Protocols: Methods in Molecular Biology (Vol. 974, pp. 1-21). https://doi.org/10.1007/978-1-62703-275-9_1

Jensen, P. W., Falconi, M., Kristoffersen, E. L., Simonsen, A. T., Cifuentes, J. B., Marcussen, L., Hougaard, R. F., Vagner, J., Harmsen, C., Jensen, S. J., Ho, Y.-P., Withers, M. A., Lupski, J. R., Koch, J. E., Desideri, A., Knudsen, B. R. & Stougaard, M. (2013). Real-time detection of TDP1 activity using a fluorophore-quencher coupled DNA-biosensor. Biosensors and Bioelectronics, Volume 48, 230–237. https://doi.org/10.1016/j.bios.2013.04.019

Farjami, E., Campos, R., Nielsen, J. S., Gothelf, K. V., Kjems, J. & Ferapontova, E. (2013). RNA aptamer-based electrochemical biosensor for selective and label-free analysis of dopamine. Analytical Chemistry, 85(1), 121-128. https://doi.org/10.1021/ac302134s

Beerens, N., Jepsen, M. D. E., Nechyporuk-Zloy, V., Krüger, A. C., Darlix, J.-L., Kjems, J. & Birkedal, V. (2013). Role of the primer activation signal in tRNA annealing onto the HIV-1 genome studied by single-molecule FRET microscopy. RNA, 19(4), 517-26. https://doi.org/10.1261/rna.035733.112

Nielsen, K. H. (2013). Samspil mellem videnskab, politik og myndigheder. Videnskab.dk. http://videnskab.dk/blog/samspil-mellem-videnskab-politik-og-myndigheder

Nielsen, K. H. (2013). Science journalism in the twentieth century: exploring the boundaries between science news, science advocacy and fourth-estate science journalism. Abstract from Knowledge at work, Manchester, United Kingdom. http://www.ichstm2013.com/programme/guide/p/1125.html

Nielsen, K. H. (2013). Scientific Communication and the Nature of Science. Science & Education, 22(9), 2067-2086. https://doi.org/10.1007/s11191-012-9475-3

Quinones, J. P., Gothelf, K. V., Kjems, J., Yang, C., Caballero, A. M. H., Schmidt, C. & Peniche Covas, C. (2013). Self-assembled nanoparticles of modified-chitosan conjugates for the sustained release of dl-α-tocopherol. Carbohydrate Polymers, 92(1), 856-864. https://doi.org/10.1016/j.carbpol.2012.10.005

Zhang, Z., Song, J., Besenbacher, F., Dong, M. & Gothelf, K. V. (2013). Self-Assembly of DNA Origami and Single-Stranded Tile Structures at Room Temperature. Angewandte Chemie International Edition, 52(35), 9219-9223. https://doi.org/10.1002/anie.201303611

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Kalinka Hansen