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Recent research news on Artificial Biology

27 November 2023 - Research news

In this work, synthetic cells equipped with an artificial signaling pathway that connects an extracellular trigger event to the activation of intracellular transcription are engineered. Learning from nature, this is done via an engineering of responsive enzymes, such that activation of enzymatic activity can be triggered by an external biochemical stimulus. Reversibly deactivated creatine kinase to achieve triggered production of adenosine triphosphate, and a reversibly deactivated nucleic acid…

01 May 2023 - Research news

Nanopores are label-free single-molecule analytical tools that show great potential for stochastic sensing of proteins. Here, we described a ClyA nanopore functionalized with different nanobodies through a 5–6 nm DNA linker at its periphery. Ty1, 2Rs15d, 2Rb17c, and nb22 nanobodies were employed to specifically recognize the large protein SARS-CoV-2 Spike, a medium-sized HER2 receptor, and the small protein murine urokinase-type plasminogen activator (muPA), respectively. The pores modified with…

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Kim, J.-Y., Sahu, S., Yau, Y.-H., Wang, X., Shochat, S. G., Nielsen, P. H., Dueholm, M. S., Otzen, D. E., Lee, J., Delos Santos, M. M. S., Yam, J. K. H., Kang, N.-Y., Park, S.-J., Kwon, H., Seviour, T. W., Yang, L., Givskov, M. & Chang, Y.-T. (2016). Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11. Journal of the American Chemical Society, 138(1), 402-407. https://doi.org/10.1021/jacs.5b11357

Jordal, P. L., Dyrlund, T. F., Winge, K., Larsen, M. R., Danielsen, E. H., Wells, J. A., Otzen, D. E. & Enghild, J. J. (2016). Detection of proteolytic signatures for Parkinson's disease. Future Neurology, 11(1), 15-32. https://doi.org/10.2217/fnl.16.3

Wang, J., Zhu, Z., Bortolini, C., Hoffmann, S. V., Amari, A., Zhang, H. X., Liu, L. & Dong, M. D. (2016). Dimensionality of carbon nanomaterial impacting on the modulation of amyloid peptide assembly. Nanotechnology, 27(30), Article 304001. https://doi.org/10.1088/0957-4484/27/30/304001

Nielsen, K. H. (2016). Diskurs. Weekendavisen, Ideer, 10.

Nielsen, K. H. (2016). Dna. Weekendavisen, Ideer, 10.

Okholm, A. H. & Kjems, J. (2016). DNA nanovehicles and the biological barriers. Advanced Drug Delivery Reviews, 106(Part A), 183-191. Article 16. https://doi.org/10.1016/j.addr.2016.05.024

Kodal, A. L. B., Rosen, C. B., Mortensen, M. R., Tørring, T. & Gothelf, K. V. (2016). DNA-Templated Introduction of an Aldehyde Handle in Proteins. ChemBioChem, 17(14), 1338-1342. https://doi.org/10.1002/cbic.201600254

Armada-Moreira, A., Taipaleenmäki, E. M., Itel, F., Zhang, Y. & Stadler, B. M. (2016). Droplet-microfluidics towards the assembly of advanced building blocks in cell mimicry. Nanoscale, 8(47), 19510-19522. https://doi.org/10.1039/C6NR07807A

Nielsen, C. K., Kjems, J., Mygind, T., Snabe, T. & Meyer, R. L. (2016). Effects of Tween 80 on Growth and Biofilm Formation in Laboratory Media. Frontiers in Microbiology, 7(NOV), Article 1878. https://doi.org/10.3389/fmicb.2016.01878

Nielsen, C. K., Kjems, J., Mygind, T., Snabe, T., Schwarz, K., Serfert, Y. & Meyer, R. L. (2016). Enhancing the antibacterial efficacy of isoeugenol by emulsion encapsulation. International Journal of Food Microbiology, 229, 7-14. https://doi.org/10.1016/j.ijfoodmicro.2016.04.002

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