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Recent research news on Artificial Biology

27 November 2023 - Research news

In this work, synthetic cells equipped with an artificial signaling pathway that connects an extracellular trigger event to the activation of intracellular transcription are engineered. Learning from nature, this is done via an engineering of responsive enzymes, such that activation of enzymatic activity can be triggered by an external biochemical stimulus. Reversibly deactivated creatine kinase to achieve triggered production of adenosine triphosphate, and a reversibly deactivated nucleic acid…

01 May 2023 - Research news

Nanopores are label-free single-molecule analytical tools that show great potential for stochastic sensing of proteins. Here, we described a ClyA nanopore functionalized with different nanobodies through a 5–6 nm DNA linker at its periphery. Ty1, 2Rs15d, 2Rb17c, and nb22 nanobodies were employed to specifically recognize the large protein SARS-CoV-2 Spike, a medium-sized HER2 receptor, and the small protein murine urokinase-type plasminogen activator (muPA), respectively. The pores modified with…

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Nørnberg, P., Thøgersen, J., Bak, E. N., Finster, K., Jakobsen, H. J. & Jensen, S. K. (2019). Saltation may be contributory in the depletion of methane on Mars. EGU General Assembly (EGU2019), Proceedings, 21(13986), 201.

Nielsen, K. H. (2019). Science of science communication. Metascience, 28(1), 85-87. https://doi.org/10.1007/s11016-018-0375-2

Liu, T., Song, P., Märcher, A., Kjems, J., Yang, C. & Gothelf, K. V. (2019). Selective delivery of doxorubicin to EGFR+ cancer cells by Cetuximab‐DNA conjugates. ChemBioChem, 20(8), 1014-1018. https://doi.org/10.1002/cbic.201800685

Jepsen, M. D. E., Sørensen, R. S., Maffeo, C., Aksimentiev, A., Kjems, J. & Birkedal, V. (2019). Single molecule analysis of structural fluctuations in DNA nanostructures. Nanoscale, 11(39), 18475-18482. https://doi.org/10.1039/c9nr03826d

Consiglieri, E., Xu, Q., Bregnhøj, M., Westberg, M., Ogilby, P. R. & Losi, A. (2019). Single mutation in a novel bacterial LOV protein yields a singlet oxygen generator. Photochemical & Photobiological Sciences, 18(11), 2657-2660. https://doi.org/10.1039/C9PP00328B

Ade, C. A., Brodszkij, E., Thingholm, B., Gal, N., Itel, F. R., Taipaleenmäki, E. M., Schattling, P. & Stadler, B. M. (2019). Small Organic Catalase Mimic Encapsulated in Micellar Artificial Organelles as Reactive Oxygen Species Scavengers. ACS Applied Polymer Materials, 1(6), 1532-1539. https://doi.org/10.1021/acsapm.9b00308

Verboom, K., Everaert, C., Bolduc, N., Livak, K. J., Yigit, N., Rombaut, D., Anckaert, J., Lee, S., Venø, M. T., Kjems, J., Speleman, F., Mestdagh, P. & Vandesompele, J. (2019). SMARTer single cell total RNA sequencing. Nucleic Acids Research, 47(16), Article e93. https://doi.org/10.1093/nar/gkz535

Hornekær, L. (2019). Stabilizing a C-H bond on graphene with sound. Science (New York, N.Y.), 364(6438), 331-332. https://doi.org/10.1126/science.aax1980

Kristoffersen, E. L. & Birkedal, V. (2019). Stable Off-Path Structures in the Folding Dynamics of Two Consecutive Telomeric DNG G-Quadruplexes. 138A-138A. https://doi.org/10.1016/j.bpj.2018.11.764

Cui, H., Gu, Z., Chen, X., Lin, L., Wang, Z., Dai, X., Yang, Z., Liu, L., Zhou, R. & Dong, M. (2019). Stimulating antibacterial activities of graphitic carbon nitride nanosheets with plasma treatment. Nanoscale, 11(39), 18416-18425. https://doi.org/10.1039/c9nr03797g

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