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Recent research news on Artificial Biology

27 November 2023 - Research news

In this work, synthetic cells equipped with an artificial signaling pathway that connects an extracellular trigger event to the activation of intracellular transcription are engineered. Learning from nature, this is done via an engineering of responsive enzymes, such that activation of enzymatic activity can be triggered by an external biochemical stimulus. Reversibly deactivated creatine kinase to achieve triggered production of adenosine triphosphate, and a reversibly deactivated nucleic acid…

01 May 2023 - Research news

Nanopores are label-free single-molecule analytical tools that show great potential for stochastic sensing of proteins. Here, we described a ClyA nanopore functionalized with different nanobodies through a 5–6 nm DNA linker at its periphery. Ty1, 2Rs15d, 2Rb17c, and nb22 nanobodies were employed to specifically recognize the large protein SARS-CoV-2 Spike, a medium-sized HER2 receptor, and the small protein murine urokinase-type plasminogen activator (muPA), respectively. The pores modified with…

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Andersen, J. P., Sørensen, T. L.-M., Povlsen, K. & Vilsen, B. (2001). Importance of transmembrane segment M3 of the sarcoplasmic reticulum Ca²⁺-ATPase for control of the gateway to the Ca²⁺-sites. Journal of Biological Chemistry, 276, 23312-23321.

Dirac, A. G. & Kjems, J. (2001). Mapping DNA sites of HIV-1 integrase by protein footprinting. FEBS journal, 268, 743-751.

Skovhus, T. L. & Finster, K. (2001). Når bakterier snakker. Aktuel Naturvidenskab, 2, 4-7.

Verhoef, K., Bilodeau, P. S., nullWamelJ.L.B., V., Kjems, J., Stoltzfus, C. M. & Berkhout, B. (2001). Repair of a Rev-minus HIV-1 mutant by activation of a cryptic splice site. J. Virol., 75, 3495-3500.

de Wit, R., Stal, L. J., Lomstein, B. A., Herbert, R. A., van Gemerden, H., Viaroli, P., Cecherelli, V.-U., Rodrigues-Valera, F., Bartoli, M., Giordani, G., Azzoni, R., Schaub, B., Welsh, T. D., Donelly, A., Cifuentes, A., Antón, J., Finster, K., Nielsen, L. B., Pedersen, A.-GU. ... Heijs, S. K. (2001). ROBUST: The ROle of BUffering capacities in STabilising Coastal lagoon ecosystems. Continental Shelf Research, 21, 2021-2041. http://www.elsevier.com/locate/csr

Tange, T. Ø. & Kjems, J. (2001). SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain. J. Mol. Biol., 312, 649-662.

Hornekær, L., Kjærggard, N., Thommesen, A. M. & Drewsen, M. (2001). Structural properties of two-component Coulomb crystals in linear Paul traps. Physical Review Letters, 86, 1994.

Nielsen, L. B., Finster, K., Welsh, D. T., Donelly, A., Herbert, R. A., de Wit, R. & Lomstein, B. A. (2001). Sulphate reduction and nitrogen fixation rates associated with roots, rhizomes and sediments from Zostera noltii and Spartina maritima meadows. Environmental Microbiology, 3(1), 63-71.

Brodal, G. S., Fagerberg, R., Pedersen, C. N. S. & Östlin, A. (2001). The Complexity of Constructing Evolutionary Trees Using Experiments. In F. Orejas, P. G. Spirakis & J. van Leeuwen (Eds.), Automata, Languages and Programming: 28th International Colloquium, ICALP 2001 Crete, Greece, July 8–12, 2001 Proceedings (pp. 140-151). Springer. https://doi.org/10.1007/3-540-48224-5_12

Brodal, G. S., Fagerberg, R., Pedersen, C. N. S., Östlin, A., Orejas, F. (Ed.), Spirakis, P. G. (Ed.) & Leeuwen, J. V. (Ed.) (2001). The Complexity of Constructing Evolutionary Trees Using Experiments. In Lecture Notes In Computer Science; Vol. 2076: Proceedings of the 28th International Colloquium on Automata, Languages and Programming (2076 of Lecture Notes in Computer Science ed., Vol. 2076/2001, pp. 140-151). Springer.

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