Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Bally, M., Bailey, K., Sugihara, K., Grieshaber, D., Voros, J. & Stadler, B. (2010). Liposome and Lipid Bilayer Arrays Towards Biosensing Applications. Small, 6(22), 2481-2497.

Kartashov, A. V., Serafini, G., Dong, M., Shipovskov, S., Gazaryan, I., Besenbacher, F. & Ferapontova, E. (2010). Long-range electron transfer in recombinant peroxidases anisotropically orientated on gold electrodes. Physical Chemistry Chemical Physics, 12, 10098-10107. https://doi.org/10.1039/c0cp00605j

Ferapontova, E., Kartashov, A., Dong, M., Shipovskov, S. & Besenbacher, F. (2010). Long-range electron transfer in recombinant peroxidases anisotropically oriented on gold. Abstract from The 61st Annual Meeting of the International Society of Electrochemistry, Nice, France.

Ferapontova, E., Kartashov, A., Dong, M., Shipovskov, S. & Besenbacher, F. (2010). Long-range electron transfer in recombinant peroxidases anisotropically oriented on gold. Poster session presented at The 61st Annual Meeting of the International Society of Electrochemistry, Nice, France.

Nielsen, K. H. (2010). Mark B. Brown: Science in Democracy: Expertise, Institutions, and Representation: The MIT Press: Cambridge, Mass., and London, UK 2009. Science Studies, 23(1), 96-98. http://www.sciencestudies.fi/system/files/Book+review+1.pdf

Basse-O'Connor, A., Graversen, S.-E. & Pedersen, J. (2010). Martingale-type processes indexed by the real line. Alea, 7, 117-137. http://alea.impa.br/articles/v7/07-06.pdf

Rotaru, A.-E., Skrydstrup, T., Finster, K. & Meyer, R. L. (2010). Mechanism of Pd (II) reduction on microbial and synthetic surfaces. Poster session presented at ISME 13 - 13th International Symposium on Microbial Ecology, Seattle, WA, United States.

Rotaru, A.-E., Skrydstrup, T., Finster, K. & Meyer, R. L. (2010). Mechanism of Pd(II) reduction on microbial and synthetic surfaces. Poster session presented at Mechanism of Pd(II) reduction on microbial and synthetic surfaces.

Dorosz, J., Gofman, Y., Kolusheva, S., Otzen, D., Ben-Tal, N., Nielsen, N. C. & Jelinek, R. (2010). Membrane Interactions of Novicidin, a Novel Antimicrobial Peptide: Phosphatidylglycerol Promotes Bilayer Insertion. Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces & Biophysical, 114(34), 11053-60. https://doi.org/10.1021/jp1052248

Barcena, T. G., Yde, J. C. & Finster, K. (2010). Methane flux and high-affinity methanotrophic diversity along the chronosequence of a receding glacier in Greenland. Annals of Glaciology, 51(56), 23-31.

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