Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Bonnet, G., Hirsch, C., Rosen, D. & Willhalm, D. (2023). Limit theory of sparse random geometric graphs in high dimensions. Stochastic Processes and Their Applications, 163, 203-236. https://doi.org/10.1016/j.spa.2023.06.002

Zhang, D., Huang, M., Klausen, L. H., Li, Q., Li, S. & Dong, M. (2023). Liquid-Phase Friction of Two-Dimensional Molybdenum Disulfide at the Atomic Scale. ACS Applied Materials & Interfaces, 15(17), 21595-21601. https://doi.org/10.1021/acsami.3c00221

Nielsen, K. H. (2023). Magisk tænkning. Weekendavisen, Sektion 4 (Ideer), 5.

Kristensen, M. M., Løvschall, K. B. & Zelikin, A. N. (2023). Mechanisms of Degradation for Polydisulfides: Main Chain Scission, Self-Immolation, Or Chain Transfer Depolymerization. ACS Macro Letters, 12(7), 955-960. https://doi.org/10.1021/acsmacrolett.3c00345

Klein, V. J., Brito, L. F., Perez-Garcia, F., Brautaset, T. & Irla, M. (2023). Metabolic engineering of thermophilic Bacillus methanolicus for riboflavin overproduction from methanol. Microbial Biotechnology, 16(5), 1011-1026. https://doi.org/10.1111/1751-7915.14239

Terlouw, B. R., Blin, K., Navarro-Muñoz, J. C., Avalon, N. E., Chevrette, M. G., Egbert, S., Lee, S., Meijer, D., Recchia, M. J. J., Reitz, ZL., van Santen, JA., Selem-Mojica, N., Tørring, T., Zaroubi, L., Alanjary, M., Aleti, G., Aguilar, C., Al-Salihi, S. A. A., Augustijn, HE. ... Medema, MH. (2023). MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters. Nucleic Acids Research, 51(D1), D603-D610. Article gkac1049. https://doi.org/10.1093/nar/gkac1049

de Dios Andres, P. & Städler, B. (2023). Micromotor-Assisted Keratinocytes Migration in a Floating Paper Chip. Small, 19(13), Article 2201251. https://doi.org/10.1002/smll.202201251

Heiland, M., Connolly, N. M. C., Mamad, O., Nguyen, N. T., Kesavan, J. C., Langa, E., Fanning, K., Sanfeliu, A., Yan, Y., Su, J., Venø, M. T., Costard, L. S., Neubert, V., Engel, T., Hill, T. D. M., Freiman, T. M., Mahesh, A., Tiwari, V. K., Rosenow, F. ... Henshall, D. C. (2023). MicroRNA-335-5p suppresses voltage-gated sodium channel expression and may be a target for seizure control. Proceedings of the National Academy of Sciences (PNAS), 120(30), e2216658120. Article e2216658120. https://doi.org/10.1073/pnas.2216658120

Nielsen, K. H. (2023). Misinformation om løgne. Weekendavisen, Sektion 4 (Ideer), 5.

Pelizzola, M., Laursen, R. & Hobolth, A. (2023). Model selection and robust inference of mutational signatures using Negative Binomial non-negative matrix factorization. BMC Bioinformatics, 24(1), Article 187. https://doi.org/10.1186/s12859-023-05304-1

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