Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Andersen, F. F., Tange, T. Ø., Sinnathamby, T., Olesen, J. R., Andersen, K. E., Westergaard, O., Kjems, J. & Knudsen, B. R. (2002). The RNA splicing factor ASF/SF2 inhibits human topoisomerase I mediated DNA relaxation. Journal of Molecular Biology.

Nielsen, K. H. (2002). Tilting at windmills: On Actor-Worlds, Socio-Logics, and Techno-Economic Networks of Wind Power, 1974-1999.

Guldberg, L. B., Finster, K., Jørgensen, N. O. G., Middelboe, M. & Lomstein, B. A. (2002). Utilization of marine sedimentary dissolved organic nitrogen by native anaerobic bacteria. Limnology and Oceanography, 47(6), 1712-1722.

Bot, A. N. M., Ortius-Lechner, D., Finster, K. & Boomsma, J. J. (2002). Variable sensitivity of fungi and bacteria to compounds produced by the metapleural glands of leaf-cutting ants. Insectes soc., 49, 363-370.

Nilsson, J., Askjaer, P. & Kjems, J. (2001). A role for the basic patch and the C-terminus of RanGTP in regulating the dynamic interaction with importin β, CRM1, and RanBP1. J. Mol. Biol., 305, 231-243.

Thomsen, T. R., Finster, K. & Ramsing, N. B. (2001). Biogeochemical and Molecular Signatures of Anaerobic Methane Oxidation in a Marine Sediment. Applied and Environmental Microbiology, 67(4), 1646-1656.

Jagota, A., Lyngsø, R. B., Pedersen, C. N. S., Gascuel, O. (Ed.) & Moret, B. M. E. (Ed.) (2001). Comparing Hidden Markov Model and a Stochastic Context-Free Grammer. In Proceedings of the First International Workshop on Algorithms in Bioinformatics (2149 of Lecture Notes in Computer Science ed., Vol. Volume 2149/2001, pp. 69-94). Springer.

Lyngsø, R. B., Pedersen, C. N. S., Eades, P. (Ed.) & Takaoka, T. (Ed.) (2001). Complexity of Comparing Hidden Markov Models. In Ikke angivet (2223 of Lecture Notes in Computer Science ed., pp. 416-428). Springer.

Brodal, G. S., Fagerberg, R., Pedersen, C. N. S., Eades, P. (Ed.) & Takaoka, T. (Ed.) (2001). Computing the Quartet Distance Between Evolutionary Trees in Time O(n log² n). In Proceedings of Algorithms and Computation : 12th International Symposium, ISAAC 2001 (2223 of Lecture Notes in Computer Science ed., Vol. 2223/2001, pp. 731-742). Springer.

Poulsen, H., Nilsson, J., Damgaard, C. K., Jensen, J. E. & Kjems, J. (2001). CRM1 mediates the export of ADAR1 through a nuclear export signal within the Z-DNA binding domain. Molecular and Cellular Biology, 21, 7862-7871.

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