Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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Zhang, C., Peng, S., Ouyang, Y., Han, J., Li, C., Wei, Y., Jiang, Y., Dong, M. & Wang, J. (2024). Broadband self-powered MoS₂/PdSe₂/WSe₂ PSN heterojunction photodetector for high-performance optical imaging and communication. Optics Express, 32(22), 38136-38146. https://doi.org/10.1364/OE.539148

Pirhaghi, M., Mamashli, F., Moosavi-Movahedi, F., Arghavani, P., Amiri, A., Davaeil, B., Mohammad-Zaheri, M., Mousavi-Jarrahi, Z., Sharma, D., Langel, Ü., Otzen, D. E. & Saboury, A. A. (2024). Cell-Penetrating Peptides: Promising Therapeutics and Drug-Delivery Systems for Neurodegenerative Diseases. Molecular Pharmaceutics, 21(5), 2097-2117. https://doi.org/10.1021/acs.molpharmaceut.3c01167

Andersen, D. G., Pedersen, A. B., Jørgensen, M. H., Montasell, M. C., Søgaard, A. B., Chen, G., Schroeder, A., Andersen, G. R. & Zelikin, A. N. (2024). Chemical Zymogens and Transmembrane Activation of Transcription in Synthetic Cells. Advanced Materials, 36(6), Article 2309385. https://doi.org/10.1002/adma.202309385

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Okholm, T. L. H., Kamstrup, A. B., Nielsen, M. M., Hollensen, A. K., Graversgaard, M. L., Sørensen, M. H., Kristensen, L. S., Vang, S., Park, S. S., Yeo, E., Dyrskjøt, L., Kjems, J., Pedersen, J. S. & Damgaard, C. K. (2024). circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA. eLife, 13, Article RP91783. https://doi.org/10.7554/eLife.91783

Rybiczka-Tešulov, M., Garritsen, O., Venø, M. T., Wieg, L., Dijk, R. V., Rahimi, K., Gomes-Duarte, A., Wit, M. D., van de Haar, L. L., Michels, L., van Kronenburg, N. C. H., van der Meer, C., Kjems, J., Vangoor, V. R. & Pasterkamp, R. J. (2024). Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development. Nature Communications, 15(1), Article 6773. https://doi.org/10.1038/s41467-024-51041-1

Scoyni, F., Sitnikova, V., Giudice, L., Korhonen, P., Trevisan, D. M., Hernandez de Sande, A., Gomez-Budia, M., Giniatullina, R., Ugidos, I. F., Dhungana, H., Pistono, C., Korvenlaita, N., Välimäki, N. N., Kangas, S. M., Hiltunen, A. E., Gribchenko, E., Kaikkonen-Määttä, M. U., Koistinaho, J., Ylä-Herttuala, S. ... Malm, T. (2024). ciRS-7 and miR-7 regulate ischemia-induced neuronal death via glutamatergic signaling. Cell Reports, 43(3), Article 113862. https://doi.org/10.1016/j.celrep.2024.113862

Wang, N., Marcelino, T. F., Ade, C. A., Pendlmayr, S., Ramos Docampo, M. A. & Stadler, B. M. (2024). Collagenase Motors in Gelatine-Based Hydrogels. Nanoscale, 16(20), 9935-9943. https://doi.org/10.1039/d3nr05712g

Vangoor, V. R., Giuliani, G., de Wit, M., Rangel, C. K., Venø, M. T., Schulte, J. T., Gomes-Duarte, A., Senthilkumar, K., Puhakka, N., Kjems, J., de Graan, P. N. E. & Pasterkamp, R. J. (2024). Compartment-specific small non-coding RNA changes and nucleolar defects in human mesial temporal lobe epilepsy. Acta Neuropathologica, 148(1), Article 61. https://doi.org/10.1007/s00401-024-02817-8

McRae, E. K. S., Wan, C. J. K., Kristoffersen, E. L., Hansen, K., Gianni, E., Gallego, I., Curran, J. F., Attwater, J., Holliger, P. & Andersen, E. S. (2024). Cryo-EM structure and functional landscape of an RNA polymerase ribozyme. Proceedings of the National Academy of Sciences (PNAS), 121(3), e2313332121. Article e2313332121. https://doi.org/10.1073/pnas.2313332121

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