Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Nielsen, K. H. (2019). Månen. Weekendavisen, Sektion 4 (Ideer), 10.

Liu, J., Champer, J., Langmüller, A. M., Liu, C., Chung, J., Reeves, R., Luthra, A., Lee, Y. L., Vaughn, A. H., Clark, A. G. & Messer, P. W. (2019). Maximum likelihood estimation of fitness components in experimental evolution. Genetics, 211(3), 1005-1017. https://doi.org/10.1534/genetics.118.301893

Mohammad-Beigi, H., Hosseini, A., Adeli, M., Ejtehadi, M. R., Christiansen, G., Sahin, C., Tu, Z., Tavakol, M., Dilmaghani-Marand, A., Nabipour, I., Farzadfar, F., Otzen, D. E., Mahmoudi, M. & Hajipour, M. J. (2019). Mechanistic Understanding of the Interactions between Nano-Objects with Different Surface Properties and α-Synuclein. ACS Nano, 13(3), 3243-3256. https://doi.org/10.1021/acsnano.8b08983

Chen, M., Jørgensen, M. L. & Sikkersoq Kimer Nøddesbo, M. (2019). Melt Electrowritten Filter for Capturing Cells. Manuscript submitted for publication. (Patent No. 19194329.9-1120).

Nielsen, K. H. (2019). Meme. Weekendavisen, Sektion 4 (Ideer), 10.

Fang, X., Wang, Y., Wang, Z., Jiang, Z. & Dong, M. (2019). Microorganism assisted synthesized nanoparticles for catalytic applications. Energies, 12(1), Article 190. https://doi.org/10.3390/en12010190

Ramos-Docampo, M., Fernández Medina, M., Taipaleenmäki, E. M., Hovorka, O., Salgueiriño, V. S. & Stadler, B. M. (2019). Microswimmers with Heat Delivery Capacity for 3D Cell Spheroid Penetration. A C S Nano, 13(10), 12192-12205. https://doi.org/10.1021/acsnano.9b06869