Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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Tian, P., Boomsma, W., Wang, Y., Otzen, D., Jensen, M. H. & Lindorff-Larsen, K. (2015). Structure of a Functional Amyloid Protein Subunit Computed Using Sequence Variation. Journal of the American Chemical Society, 137(1), 22-25. https://doi.org/10.1021/ja5093634

Jensen, R. K., Plum, M., Tjerrild, L., Jakob, T., Spillner, E. & Andersen, G. R. (2015). Structure of the omalizumab Fab. Acta Crystallographica Section F:Structural Biology Communications, 71(Pt 4), 419-426. https://doi.org/10.1107/S2053230X15004100

Estrela, N., Franquelim, H. G., Lopes, C., Tavares, E., Macedo, J. A., Christiansen, G., Otzen, D. E. & Melo, E. P. (2015). Sucrose prevents protein fibrillation through compaction of the tertiary structure but hardly affects the secondary structure. Proteins: Structure, Function, and Bioinformatics, 83(11), 2039-2051. https://doi.org/10.1002/prot.24921

Wang, Z., Li, P., Chen, Y., Liu, J., Zhang, W., Guo, Z., Dong, MD. & Li, Y. (2015). Synthesis, characterization and electrical properties of silicon-doped graphene films. Journal of Materials Chemistry C, 3, 6301-6306. https://doi.org/10.1039/c5tc00563a

Schiølin, K. (2015). Teknologipessimisme: Træk af slægtskabet mellem den klassiske teknologifilosofi og den pessimistiske viljesmetafysik: Schopenhauer, Nietzsche, Jünger og Heidegger . Aarhus Universitet, Institut for Kommunikation og Kultur - Informationsvidenskab.

Li, D., Stansfeld, P. J., Sansom, M. S. P., Keogh, A., Vogeley, L., Howe, N., Lyons, J. A., Aragao, D., Fromme, P., Fromme, R., Basu, S., Grotjohann, I., Kupitz, C., Rendek, K., Weierstall, U., Zatsepin, N. A., Cherezov, V., Liu, W., Bandaru, S. ... Caffrey, M. (2015). Ternary structure reveals mechanism of a membrane diacylglycerol kinase. Nature Communications, 6, Article 10140. https://doi.org/10.1038/ncomms10140

Madsen, J., Pihl, R., Møller, A. H., Tranberg Madsen, A., Otzen, D. & Andersen, K. K. (2015). The anionic biosurfactant rhamnolipid does not denature industrial enzymes. Frontiers in Microbiology, 6, Article 292. https://doi.org/10.3389/fmicb.2015.00292

Nielsen, K. H. (2015). The Bohr atom bound in cloth: Textual exposition of quantum theory in popular science books, 1918-1924. In F. Aaserud & H. Kragh (Eds.), One Hundred Years of the Bohr Atom: Proceedings from a Conference (Vol. 1, pp. 141-158). Det Kongelige Danske Videnskabernes Selskab.

Zhang, P., Hou, X., He, Y., Peng, Q. & Dong, M. (2015). The effects of surface group functionalization and strain on the electronic structures of two-dimensional silicon carbide. Chemical Physics Letters, 628, 60-65. https://doi.org/10.1016/j.cplett.2015.03.031

Waltoft, B. L., Pedersen, C. B., Nyegaard, M. & Hobolth, A. (2015). The importance of distinguishing between the odds ratio and the incidence rate ratio in GWAS. BMC Medical Genetics, 16(1), 71. https://doi.org/10.1186/s12881-015-0210-1

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