Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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Besenbacher, S., Mailund, T., Westh-Nielsen, L. & Pedersen, C. S. (2004). RBT -- A tool for building refined Buneman trees. Bioinformatics, doi:10.1093/bti195, in press.

Besenbacher, S., Mailund, T., Westh-Nielsen, L. & Pedersen, C. N. S. (2004). RBT - A Tool for Building Refined Buneman Trees. Bioinformatics, 21(8), 1711-1712.

Damgaard, C. K., Andersen, E. S., Knudsen, B., Gorodkin, J. & Kjems, J. (2004). RNA interactions in the 5' region of the HIV-1 genome. Journal of Molecular Biology, 336(2), 369-79. https://doi.org/10.1016/j.jmb.2003.12.010

Andersen, E. S., Contera, S., Knudsen, B., Damgaard, C. K., Besenbacher, F. & Kjems, J. (2004). Role of the TAR hairpin in dimerization of HIV-1 RNA. Journal of Biological Chemistry, 279, 22243-22249.

Andersen, E. S., Contera, S. A., Knudsen, B., Damgaard, C. K., Besenbacher, F. & Kjems, J. (2004). Role of the trans-activation response element in dimerization of HIV-1 RNA. Journal of Biological Chemistry, 279(21), 22243-22249. https://doi.org/10.1074/jbc.M314326200

Zahler, A. M., Damgaard, C. K., Kjems, J. & Caputi, M. (2004). SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. Journal of Biological Chemistry, 279(11), 10077-10084. https://doi.org/10.1074/jbc.M312743200

Zahler, A. M., Damgaard, C. K., Kjems, J. & Caputi, M. (2004). SC35 and hnRNPs A/B binding to a juxtaposed ESE/ESS element regulates HIV-1 tat exon 2 splicing. Journal of Biological Chemistry, 279, 10077-10084.

Hannila, M.-L., Jensen, E. B. V., Hobolth, A. & Nyengaard, J. R. (2004). Shape modelling of spatial particles from planar central sections - a case study. Journal of Microscopy, 215(2), 183-190. https://doi.org/10.1111/j.0022-2720.2004.01364.x

Kristensen, D. M. & Andersen, E. S. (2004). Stamceller - kroppens egne schweizerknive. Jyllands-Posten, Indblik.

Nielsen, K. H. & Nielsen, H. (2004). The Rise of Science Journalism in Denmark. In Public Communication of Science and Technology (PCST-8). Scientific Knowledge and Cultural Diversity. Conference Proceedings

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