Single Protein-Mediated Vesicle Division

Publications

Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Nielsen, K. H. (2009). In quest of publicity: the science-media partnership of the Galathea Deep Sea Expedition from 1950 to 1952. Public Understanding of Science, 18(4), 464-480. https://doi.org/10.1177/0963662507083529

Sehgal, P., Kosaka, O., Doe, H. & Otzen, D. (2009). Interaction and stability of mixed micelle and monolayer of nonionic and cationic surfactant mixtures. Journal of Dispersion Science and Technology, 30, 1050-1058.

Rantalainen, K., Christensen, P. A., Hafrén, A., Otzen, D., Kalkkinen, N. & Mäkinen, K. (2009). Interaction of a potyviral VPg with anionic phospholipid vesicles. Virology, 395, 114-120.

Sehgal, P., Mizuki, T., Doe, H., Wimmer, R., Larsen, K. L. & Otzen, D. (2009). Interactions and Influence of a-Cyclodextrin on the Aggregation and Interfacial Properties of Mixtures of Nonionic and Zwitterionic Surfactants. Colloid and Polymer Science, 287, 1243-1252.

Krüger, A. C., Nielsen, M. M., Lützelberger, M., Raarup, M. K., Kristensen, M., Kjems, J., Besenbacher, F. & Birkedal, V. (2009). Interactions between the human telomeric G-quadruplex and hnRNP A1 studied by single-molecule FRET. Poster session presented at X Annual Linz Winter Workshop, Linz, Austria.

Nielsen, K. H. (2009). International Perspectives on the History of Danish Wind Power. In C. Benny (Ed.), Wind Power The Danish Way: From Poul la Cour to Modern Wind Turbines (pp. 60-65). The Poul la Cour Foundation.

Nielsen, J., Pedersen, C. S. & Hein, J. (2009). Investigation of a simple heuristic improving the speed of statistical alignments. Poster session presented at 17th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB) & 8th European Conference on Computational Biology (ECCB), Stockholm, Sweden.

Nesgaard, L. W., Vad, B., Christiansen, G. & Otzen, D. (2009). Kinetic partitioning between aggregation and vesicle permeabilization by modified ADan. Biochimica et Biophysica Acta. Molecular Cell Research, 1794, 84-93.

Nielsen, K. H. (2009). Klamphuggeri. Hvem definerer god forskning? Politiken, 8.

Nielsen, K. H. (2009). Lær af de russiske forandringer. Videnskab.dk.

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