Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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Okholm, K. R., Nooteboom, S. W., Vinther, J. N., Lamberti, V., Dey, S., Andersen, E. S., Zijlstra, P. & Sutherland, D. S. (2024). Single-Molecule Multivalent Interactions Revealed by Plasmon-Enhanced Fluorescence. ACS Nano, 18(52), 35429-35442. https://doi.org/10.1021/acsnano.4c12600

Qin, R., Bai, M., Yang, K., Huo, Y., Wang, Z., Tian, X., Cheng, K., Zhang, F., Yang, Y., Dong, M., Hou, S. & Liu, L. (2024). Size effect of dynamic bioactive magnetic particles on regulated isolation of tumor cells. Applied Surface Science, 660, Article 159959. https://doi.org/10.1016/j.apsusc.2024.159959

Bobbili, M. R., Görgens, A., Yan, Y., Vogt, S., Gupta, D., Corso, G., Barbaria, S., Patrioli, C., Weilner, S., Pultar, M., Jacak, J., Hackl, M., Schosserer, M., Grillari, R., Kjems, J., Andaloussi, S. E. L. & Grillari, J. (2024). Snorkel-tag based affinity chromatography for recombinant extracellular vesicle purification. Journal of Extracellular Vesicles, 13(10), Article e12523. https://doi.org/10.1002/jev2.12523

Orr, M. E., Kulasinghe, A., Kolar, G. R., Heyn, H., Plummer, J., Sommer Kristensen, L., Kjems, J., Mills, G., Garcia-Vallejo, J. J., Nijman, I. J., West, N. P. & Cox, A. (2024). Sounding an Alarm over Spatial Biology. Genetic Engineering and Biotechnology News, 44(3), 16-17. https://doi.org/10.1089/gen.44.03.07

Shi, T., Liu, Y., Wang, D., Xia, D., Li, B., Xu, R., Li, N., Liang, C. & Chen, M. (2024). Spatially engineering tri-layer nanofiber dressings featuring asymmetric wettability for wound healing. Nano Materials Science, 6(5), 611-624. https://doi.org/10.1016/j.nanoms.2024.01.008

Adam, L., Kumar, R., Arroyo-Garcia, L. E., Molenkamp, W. H., Nowak, J. S., Klute, H., Farzadfard, A., Alkenayeh, R., Nielsen, J., Biverstål, H., Otzen, D. E., Johansson, J. & Abelein, A. (2024). Specific inhibition of α-synuclein oligomer generation and toxicity by the chaperone domain Bri2 BRICHOS. Protein Science, 33(8), Article e5091. https://doi.org/10.1002/pro.5091

Žvirblis, M., Sakalauskas, A., Janvand, S. H. A., Dudutienė, V., Žiaunys, M., Sniečkutė, R., Otzen, D. E., Smirnovas, V. & Matulis, D. (2024). Structure-Activity Relationship of Fluorinated Benzenesulfonamides as Inhibitors of Amyloid-β Aggregation. Chemistry - A European Journal, 30(58), Article e202402330. https://doi.org/10.1002/chem.202402330

Nielsen, K. H. (2024). Studenteroprør og nej tak til atomkraft. In C. M. K. Pedersen & C. Koldbye (Eds.), Naturvidenskabens Ildsjæle: Selskabet for Naturlærens Udbredelse gennem 200 år (pp. 172-79). Gad.

Su, Y., Zhang, Y., Chen, Y., Majidi, S. S., Dong, M. & Chen, M. (2024). Surface recrystallization on melt electrowritten scaffolds for acceleration of osteogenic differentiation. Materials Today Physics, 41, Article 101344. https://doi.org/10.1016/j.mtphys.2024.101344

Zhang, W., Wang, J., Chen, M. & Dong, M. (2024). Sustainable synthesis: Unlocking potential with plasma-assisted conversion. Matter, 7(8), 2764-2765. https://doi.org/10.1016/j.matt.2024.06.033

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