Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Nielsen, K. H. (2023). 246 biologer. Weekendavisen, Sektion 4 (Ideer), 5.

Andersen, C. B., Lausdahl, A. K., Nielsen, J., Clausen, M. P., Mulder, F. A. A., Otzen, D. E. & Arnspang, E. C. (2023). 4-Oxo-2-nonenal-Induced α-Synuclein Oligomers Interact with Membranes in the Cell, Leading to Mitochondrial Fragmentation. Biochemistry, 62(16), 2417-2425. https://doi.org/10.1021/acs.biochem.3c00114

Seeler, S., Andersen, M. S., Sztanka-Toth, T., Rybiczka-Tešulov, M., van den Munkhof, M. H., Chang, C. C., Maimaitili, M., Venø, M. T., Hansen, T. B., Pasterkamp, R. J., Rybak-Wolf, A., Denham, M., Rajewsky, N., Kristensen, L. S. & Kjems, J. (2023). A Circular RNA Expressed from the FAT3 Locus Regulates Neural Development. Molecular Neurobiology, 60(6), 3239–3260. https://doi.org/10.1007/s12035-023-03253-7

Dieudonné, T., Kümmerer, F., Laursen, M. J., Stock, C., Flygaard, R. K., Khalid, S., Lenoir, G., Lyons, J. A., Lindorff-Larsen, K. & Nissen, P. (2023). Activation and substrate specificity of the human P4-ATPase ATP8B1. Nature Communications, 14, Article 7492. https://doi.org/10.1038/s41467-023-42828-9

Nielsen, K. H. (2023). AI: Ven eller fjende? Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2023). Akademisk mobning. Weekendavisen, Sektion 4 (Ideer), 5.

Ali, S. A., Chung, K. H. K., Forgham, H., Olsen, W. P., Kakinen, A., Balaji, A., Otzen, D. E., Davis, T. P. & Javed, I. (2023). Alzheimer's Progenitor Amyloid-β Targets and Dissolves Microbial Amyloids and Impairs Biofilm Function. Advanced Science, 10(29), Article e2301423. https://doi.org/10.1002/advs.202301423

Zhang, L., Zhang, P., Wu, R., Han, A., Cheng, K., Wang, Z., Yang, J., Hou, S., Tong, Y., Dong, M. & Liu, L. (2023). Amyloid fibrils with charge transportation facilitating the green synthesis of single-crystalline gold nanoplates for catalytic application. Applied Surface Science, 622, Article 156937. https://doi.org/10.1016/j.apsusc.2023.156937

Feng, Y., Cheng, Z., Larsen, A. K. K., Shi, H., Sun, T., Dong, M. & Liu, L. (2023). Amyloid-like nanofibrous network confined and aligned ultrafine bimetallic nanozymes for smart antibacterial therapy. Materials Today Bio, 22, Article 100730. https://doi.org/10.1016/j.mtbio.2023.100730

Olsen, M., Andersen, L. N. & Gross, A. (2023). An asymptotically optimal algorithm for online stacking. Mathematical Methods of Operations Research, 97(2), 161-178. https://doi.org/10.1007/s00186-022-00808-7

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