Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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Valero, J. & Famulok, M. (2020). Regeneration of Burnt Bridges on a DNA Catenane Walker. Angewandte Chemie - International Edition, 59(38), 16366-16370. https://doi.org/10.1002/anie.202004447

Kania, K., Colella, F., Riemen, A. H. K., Wang, H., Howard, K., Aigner, T., Dell’Accio, F., Capellini, T. D., Roelofs, A. J. & De Bari, C. (2020). Regulation of Gdf5 expression in joint remodelling, repair and osteoarthritis. Scientific Reports, 10(1), 157. Article 157. https://doi.org/10.1038/s41598-019-57011-8

Godskesen, L. E., Lassen, T. R., Jespersen, N. R., Siersbæk, M., Yan, Y., Nielsen, M. M., Tjønnfjord, S. K., Grøntved, L., Madsen, G., Kjems, J., Bøtker, H. E., Schmidt, M. R., Krag, A. & Kjeldsen, J. (2020). Remote ischemic conditioning in active ulcerative colitis: An explorative randomized clinical trial. Scientific Reports, 10(1), Article 9537. https://doi.org/10.1038/s41598-020-65692-9

Pedersen, S. L., Huynh, T. H., Pöschko, P., Fruergaard, A. S., Jarlstad Olesen, M. T., Chen, Y., Birkedal, H., Subbiahdoss, G., Reimhult, E., Thøgersen, J. & Zelikin, A. N. (2020). Remotely Triggered Liquefaction of Hydrogel Materials. ACS Nano, 14(7), 9145-9155. https://doi.org/10.1021/acsnano.0c04522

Bailey, M. H., Meyerson, W. U., Dursi, L. J., Wang, L. B., Dong, G., Liang, W. W., Weerasinghe, A., Li, S., Kelso, S., Akbani, R., Anur, P., Bailey, M. H., Buchanan, A., Chiotti, K., Covington, K., Creason, A., Ding, L., MC3 Working Group, PCAWG novel somatic mutation calling methods working group & PCAWG Consortium (2020). Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples. Nature Communications, 11(1), Article 4748. https://doi.org/10.1038/s41467-020-18151-y

Wang, Z., Wu, H. H., Li, Q., Besenbacher, F., Li, Y., Zeng, X. C. & Dong, M. (2020). Reversing Interfacial Catalysis of Ambipolar WSe₂ Single Crystal. Advanced Science, 7(3), Article 1901382. https://doi.org/10.1002/advs.201901382

Schiølin, K. (2020). Revolutionary dreams: Future essentialism and the sociotechnical imaginary of the fourth industrial revolution in Denmark. Social Studies of Science, 50(4), 542-566. https://doi.org/10.1177/0306312719867768

Nielsen, K. H. (2020). Science communication from the perspective of STS. In A. Turanlı, A. U. Aydınoğlu & M. Şahinol (Eds.), Türkiye’de STS: bilim ve teknoloji Çalışmalarına giriş (pp. 37-47). İstanbul Teknik Üniversitesi. http://hdl.handle.net/11527/18483

Krainer, G., Hartmann, A., Bogatyr, V., Nielsen, J., Schlierf, M. & Otzen, D. E. (2020). SDS-induced multi-stage unfolding of a small globular protein through different denatured states revealed by single-molecule fluorescence. Chemical Science, 11(34), 9141-9153. https://doi.org/10.1039/d0sc02100h

Li, C. H., Prokopec, S. D., Sun, R. X., Yousif, F., Schmitz, N., PCAWG Tumour Subtypes and Clinical Translation, Boutros, P. C. & PCAWG Consortium (2020). Sex differences in oncogenic mutational processes. Nature Communications, 11, Article 4330. https://doi.org/10.1038/s41467-020-17359-2

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