Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Kristensen, T. G. & Pedersen, C. S. (2009). Optimal Overlay of Ligands with Flexible Bonds Using Differential Evolution. In J. Zhang, G.-Z. Li & J. Y. Yang (Eds.), Proceedings of the 2009 International Joint conferences on Bioinformatics, System biology and Intelligent Computing (IJCBS2009) (pp. 331-334). IEEE Computer Society Press. https://doi.org/10.1109/IJCBS.2009.36

Pedersen, C. S. & Kristensen, T. G. (2009). Optimal Overlay of Ligands with Flexible Bonds Using Differential Evolution. Poster session presented at 17th Annual International Conference on Intelligent Systems for Molecular Bioligy (ISMB) & 8th European Conference on Computational Biology (ECCB), Stockholm, Sweden. http://www.iscb.org/ismbeccb2009/poster_list.php?cat=C

Nielsen, K. H. (2009). På jagt efter søslangen: Galathea-ekspeditionen 1950-52. Aarhus Universitetsforlag.

Wang, J. G., Lv, Y. A., Li, X. N. & Dong, M. D. (2009). Point-Defect Mediated Bonding of Pt Clusters on (5,5) Carbon Nanotubes. Journal of Physical Chemistry Part C: Nanomaterials and Interfaces, 113(3).

Nygaard, J. V., Andersen, M. Ø., Cloetens, P., Foss, M., Kassem, M., Kjems, J. & Besenbacher, F. (2009). Polycaprolactone nanomesh cultured with hMSC evaluated by synchrotron tomography. Poster session presented at Self-assembly: from nature to clinics, Porto, Portugal.

Sneppen, K., Lizana, G. L., Jensen, M. H., Pigolotti, S. & Otzen, D. (2009). Proteasome-Fibrillation Antagonism in a Dynamic Model for Parkinson’s Disease. Physical Biology, 6, 36005.

Kilic, N., Stensballe, A., Otzen, D. & Dönmez, G. (2009). Proteomic changes in response to chromium(VI) toxicity in Pseudomonas aeruginosa . Bioresource Technology, 101, 2134-2140.

Franzmann, M., Otzen, D. & Wimmer, R. (2009). Quantitative use of paramagnetic relaxation enhancements for determining orientation and insertion depth of peptides in micelles. ChemBioChem, 10, 2339-2347.

Hansen, A. A., Jensen, L. L., Kristoffersen, T., Mikkelsen, K. A., Merrison, J. P., Finster, K. & Lomstein, B. A. (2009). Research Article: Effects of long-term simulated Martian conditions on a freeze-dried and homogenized bacterial permafrost community. Astrobiology, 9(2). https://doi.org/10.1089/ast.2008.0244

Andersen, M. Ø., Howard, K. A. & Kjems, J. (2009). RNAi using a chitosan/siRNA nanoparticle system: in vitro and in vivo applications. In Methods in Molecular Biology (Vol. 555, pp. 77-86) https://doi.org/10.1007/978-1-60327-295-7_6

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