Single Protein-Mediated Vesicle Division

Publications

Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

Sort by: Date | Author | Title

Nielsen, K. H. (2023). Hitler-billen. Weekendavisen, 5.

Kaarsted, T., Blake, O., Nielsen, K. H., Alving, B., Rasmussen, L. T., Denmark, U. & Hansen, S. M.-B. (2023). How European Research Libraries Can Support Citizen-Enhanced Open Science. Open Information Science, 7(1), Article 20220146. https://doi.org/10.1515/opis-2022-0146

Wu, R., Wang, Z., Jia, Z., Li, C., Wang, J., Liu, L. & Dong, M. (2023). Identification of hybrid amyloid strains assembled from amyloid-β and human islet amyloid polypeptide. Nanotechnology, 34(50), Article 505101. https://doi.org/10.1088/1361-6528/acf3ee

Busk, M., Eggertsen, P. P., Overgaard, J., Horsman, M. R., Tørring, T., Jacobsen, K. M. & Poulsen, T. B. (2023). In Vitro Characterization of the Bacteria-derived Hypoxia-selective Cytotoxin BE-₄₃₅₄₇. Anticancer Research, 43(12), 5319-5329. https://doi.org/10.21873/anticanres.16735

Wedge, E., Ahmadov, U., Hansen, T. B., Gao, Z., Tulstrup, M., Côme, C., Nonavinkere Srivatsan, S., Ahmed, T., Jespersen, J. S., Schlotmann, B. C., Schöllkopf, C., Raaschou-Jensen, K., Ødum, N., Kjems, J., Bak, R. O., Walter, M. J., Grønbæk, K. & Kristensen, L. S. (2023). Impact of U2AF1 mutations on circular RNA expression in myelodysplastic neoplasms. Leukemia, 37(5), 1113-1125. https://doi.org/10.1038/s41375-023-01866-4

Amin, H., Šantl-Temkiv, T., Cramer, C., Finster, K., Real, F. G., Gislason, T., Holm, M., Janson, C., Jögi, N. O., Jogi, R., Malinovschi, A., Marshall, I. P. G., Modig, L., Norbäck, D., Shigdel, R., Sigsgaard, T., Svanes, C., Thorarinsdottir, H., Wouters, I. M. ... Bertelsen, R. J. (2023). Indoor Airborne Microbiome and Endotoxin: Meteorological Events and Occupant Characteristics Are Important Determinants. Environmental Science & Technology, 57(32), 11750-11766. https://doi.org/10.1021/acs.est.3c01616

Nielsen, K. H. (2023). Inficeret med misinformation. Weekendavisen, Sektion 4 (Ideer), 13.

Hirsch, C., Holmes, M. & Kleptsyn, V. (2023). Infinite WARM graphs III: Strong reinforcement regime. Nonlinearity, 36(6), 3013-3042. https://doi.org/10.1088/1361-6544/acc9a0

Kristoffersen, E. L., Coletta, A., Lund, L. M., Schiøtt, B. & Birkedal, V. (2023). Inhibited complete folding of consecutive human telomeric G-quadruplexes. Nucleic Acids Research, 51(4), 1571-1582. https://doi.org/10.1093/nar/gkad004

Alijanvand, S. H., Ladefoged, L. K., Zubrienė, A., Sakalauskas, A., Christiansen, G., Dudutiene, V., Schiøtt, B., Matulis, D., Smirnovas, V. & Otzen, D. E. (2023). Inhibitory effects of fluorinated benzenesulfonamides on insulin fibrillation. International Journal of Biological Macromolecules, 227, 590-600. https://doi.org/10.1016/j.ijbiomac.2022.12.105

Displaying results 461 to 470 out of 3464