Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Sørensen, T. L.-M., Dupont, Y., Vilsen, B. & Andersen, J. P. (2000). Fast kinetic analysis of conformational changes in mutants of the Ca²⁺-ATPase of sarcoplasmic reticulum. Journal of Biological Chemistry, 275, 5400-5408. https://doi.org/10.1074/jbc.275.8.5400

Schmidt-Kaler, F., Roos, C., Nägerl, H. C., Rohde, H., Gulde, S., Mundt, A., Lederbauer, M., Thalhammer, T., Zeiger, T., Barton, P., Hornekær, L., Reymond, G., Liebfried, D., Eschner, J. & Blatt, R. (2000). Groundstate cooling, quantum state engineering and study of decoherence of ions in Paul traps. Journal of Modern Optics, 47, 2573.

Kjems, J. & Askjær, P. (2000). HIV-1 Rev and its cellular partners. Advances in Pharmacology, 48, 251-298.

Sørensen, T. L.-M. & Andersen, J. P. (2000). Importance of stalk segment S5 for intramolecular communication in the sarcoplasmic reticulum Ca²⁺-ATPase. Journal of Biological Chemistry, 275, 28954-28961.

Kjærgaard, N., Hornekær, L., Thommesen, A. M., Videsen, Z. & Drewsen, M. (2000). Isotope selective loading of an ion trap using resonance-enhanced two-photon ionization. Applied Physics B, 71, 207.

Hobolth, A. & Jensen, E. B. V. (2000). Modelling stochastic chances in curve shape, with an application to cancer diagnostics. Advances in Applied Probability, 32(2), 344-362. https://doi.org/10.1239/aap/1013540167

Askjær, P., Rosendahl, R. & Kjems, J. (2000). Nuclear export of DEAD-box An3 protein by CRM1 is coupled to An3 helicase activity. Journal of Biological Chemistry, 275, 11657-11668.

Kjærgaard, N., Aggerholm, S., Bowe, P. D., Hornekær, L., Madsen, N., Nielsen, J. S., Schiffer, J. P., Siegfried, L. & Hangst, J. S. (2000). Recent results from laser cooling experiments in ASTRID - real-time imaging of ion beams. Nuclear Instruments & Methods in Physics Research, A, 441, 196-202.

Schiffer, J. P., Drewsen, M., Hangst, J. S. & Hornekær, L. (2000). Temperature, ordering, and equilibrium with time-dependent confining forces. Proc. Natl. Acad. Sci., 97, 10697.

Rondell, J. B., Finster, K. W. & Lomstein, B. A. (2000). Urea and DON uptake by a Lyngbya gracialis dominated microbial mat: A controlled laboratory experiment. Aquatic Microbial Ecology, 21(2), 169-175. https://doi.org/10.3354/ame021169

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