Single Protein-Mediated Vesicle Division

Publications

Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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Yan, Y., Gu, T., Christensen, S. D. K., Su, J., Lassen, T. R., Hjortbak, M. V., Lo, I., Venø, S. T., Tóth, A. E., Song, P., Nielsen, M. S., Bøtker, H. E., Blagoev, B., Drasbek, K. R. & Kjems, J. (2021). Cyclic hypoxia conditioning alters the content of myoblast-derived extracellular vesicles and enhances their cell-protective functions. Biomedicines, 9(9), Article 1211. https://doi.org/10.3390/biomedicines9091211

Otzen, D. E., Pedersen, J. N., Somavarapu, A. K., Clement, A., Ji, M., Petersen, E. H., Pedersen, J. S., Urban, S. & Schafer, N. P. (2021). Cys-labelling kinetics of membrane protein GlpG: a role for specific SDS binding and micelle changes? Biophysical Journal, 120(18), 4115-4128. https://doi.org/10.1016/j.bpj.2021.08.001

Nielsen, K. H. & Vestergård, G. L. (2021). Danmark ind i rumalderen: Ud i rummet med Andreas Mogensen. In P. A. Toft & K. H. Nielsen (Eds.), Dansk ekspeditionshistorie 1945-2020, bd.3: Kold krig, afkolonisering og nye horisonter (pp. 342-371). Gad.

Hansen, A. H., Harbsmeier, M., Pedersen, C. S., Nielsen, J. K., Toft, P. A. & Nielsen, K. H. (Eds.) (2021). Dansk Ekspeditionshistorie 1-3. Gad.

Hait, A. S., Olagnier, D., Sancho-Shimizu, V., Skipper, K. A., Helleberg, M., Larsen, S. M., Bodda, C., Moldovan, L. I., Ren, F., Brinck Andersen, N.-S., Thomsen, M. M., Freytag, M. R., Darmalinggam, S., Parkes, I., Kadekar, D. D., Rahbek, S. H., van der Horst, D., Kristensen, L. S., Eriksson, K. ... Mogensen, T. H. (2021). Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans. Science Immunology, 5(54), Article eabc2691. https://doi.org/10.1126/sciimmunol.abc2691

Nielsen, K. H. (2021). Definitionen på fupforskning. Weekendavisen, (Sektion 4 (Ideer)), 5.

Toft, P. A. & Nielsen, K. H. (2021). Den foranderlige ekspedition. In P. A. Toft & K. H. Nielsen (Eds.), Dansk Ekspeditionshistorie 1945-2020, bd. 3: Kold krig, afkolonisering og nye horisonter (pp. 6-17). Gad.

Nielsen, K. H. & Wolff, B. (2021). Den sidste ekspedition i kongens tjeneste: Korvetten Galatheas jordomsejling 1845-1847. In A. H. Hansen & M. Harbsmeier (Eds.), Dansk Ekspeditionshistorie 1600-1850: I kongens og oplysningens tjeneste (pp. 358-393). Gad.

Nielsen, K. H. (2021). Den videnskabelige metode er død. Weekendavisen, Sektion 4 (Ideer), 11-11.

Naldal, M. L. & Nielsen, K. H. (2021). De små mider og den store jord: Marie Hammers mosmideekspeditioner. In P. A. Toft & K. H. Nielsen (Eds.), Dansk ekspeditionshistorie 1945-2020, bd. 3: Kold krig, afkolonisering og nye horisonter (pp. 250-281). Gad.

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