Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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Jørgensen, M. L., Müller, C. A., Nøddesbo, M. S. K., Nadzieja, M., Zhang, Z., Su, Y., Just, J., Spindler, K.-L. G. & Chen, M. (2020). A Melt-electrowritten Filter for Capture and Culture of Circulating Colon Cancer Cells. Materials Today Bio, 6, Article 100052. https://doi.org/10.1016/j.mtbio.2020.100052

Pedersen, B. H., Malte, H., Ramløv, H. & Finster, K. (2020). A method for studying the metabolic activity of individual tardigrades by measuring oxygen uptake using microrespirometry. Journal of Experimental Biology, 223(22), Article jeb233072. https://doi.org/10.1242/jeb.233072

Hajipour, M. J., Mohammad-Beigi, H., Nabipour, I., Mahmoudi, N., Azhdarzadeh, M., Derakhshankhah, H., El Dawud, D., Mohammadinejad, R. & Otzen, D. (2020). Amyloid fibril inhibition, acceleration, or fragmentation: Are nano-based approaches advance in the right direction? Nano Today, 35(December), Article 100983. https://doi.org/10.1016/j.nantod.2020.100983

Sawada, M., Yamaguchi, K., Hirano, M., Noji, M., So, M., Otzen, D. E., Kawata, Y. & Goto, Y. (2020). Amyloid formation of α-synuclein based on the solubility- and supersaturation-dependent mechanism. Langmuir, 36(17), 4671-4681. https://doi.org/10.1021/acs.langmuir.0c00426

Rheinbay, E., Nielsen, M. M., Abascal, F., Wala, J. A., Shapira, O., Tiao, G., Hornshøj, H., Hess, J. M., Juul, R. I., Lin, Z., Feuerbach, L., Sabarinathan, R., Madsen, T., Kim, J., Mularoni, L., Shuai, S., Lanzós, A., Herrmann, C., Maruvka, Y. E. ... PCAWG Consortium (2020). Analyses of non-coding somatic drivers in 2,658 cancer whole genomes. Nature, 578(7793), 102-111. https://doi.org/10.1038/s41586-020-1965-x

Wang, Y., zhang, Y., Zhang, Z., Su, Y., Wang, Z., Dong, MD. & Chen, M. (2020). An Injectable High-conductive Bimaterial Scaffold for Neural Stimulation. Colloids and Surfaces B: Biointerfaces, 195, Article 111210. https://doi.org/10.1016/j.colsurfb.2020.111210

Kang, S., Fang, Z., He, M., Chen, M., Gao, Y., Sun, D., Liu, Y., Chen, M., Dong, M., Liu, P. & Cui, L. (2020). An instant, biocompatible and biodegradable high-performance graphitic carbon nitride. Journal of Colloid and Interface Science, 563, 336-346. https://doi.org/10.1016/j.jcis.2019.12.021

Zelikin, A. N. & Städler, B. (2020). Artificial Biology: Molecular Design and Cell Mimicry. Small, 16(27), Article 2003442. https://doi.org/10.1002/smll.202003442

Venø, M. T., Reschke, C. R., Morris, G., Connolly, N. M. C., Su, J., Yan, Y., Engel, T., Jimenez-Mateos, E. M., Harder, L. M., Pultz, D., Haunsberger, S. J., Pal, A., Heller, J. P., Campbell, A., Langa, E., Brennan, G. P., Conboy, K., Richardson, A., Norwood, B. A. ... Henshall, D. C. (2020). A systems approach delivers a functional microRNA catalog and expanded targets for seizure suppression in temporal lobe epilepsy. Proceedings of the National Academy of Sciences (PNAS), 117(27), 15977-15988. https://doi.org/10.1073/pnas.1919313117

Jia, Y., Xiong, X., Wang, D., Duan, X., Sun, K., Li, Y., Zheng, L., Lin, W., Dong, M., Zhang, G., Liu, W. & Sun, X. (2020). Atomically Dispersed Fe-N₄ Modified with Precisely Located S for Highly Efficient Oxygen Reduction. Nano-Micro Letters, 12(1), Article 116. https://doi.org/10.1007/s40820-020-00456-8

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