Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Nesgaard, L. W., Hoffmann, S. V., Andersen, C. B., Malmendal, A. & Otzen, D. (2008). Characterization of Dry Globular Proteins and Protein Fibrils by Synchrotron Radiation Vacuum UV Circular Dichroism. Biopolymers, 89(9), 779-795.

Hein, S., Wang, K., Stevens, W. & Kjems, J. (2008). Chitosan composites forbiomedical applications: status, challenges and perspectives. Materials Science and Technology, 24, 1053-1061. https://doi.org/10.1179/174328408X341744

Howard, K. A., Paludan, S. R., Behlke, M. A., Besenbacher, F., Deleuran, B. W. & Kjems, J. (2008). Chitosan/siRNA nanoparticle-mediated TNF-alpha knockdown in peritoneal macrophages for anti-inflammatory treatment in a murine arthritis model. Molecular Therapy, 17(1), 162-168. https://doi.org/10.1038/mt.2008.220

Nielsen, K. H. (2008). Communicating knowledge in science, science journalism and art: The case of Gerhard Richter's Erster Blick (2000). Abstract from Encompassing knowledge: 2nd International Conference of the 360º conference series, Århus, Denmark.

Torarinsson, E., Yao, Z., Wiklund, E. D., Bramsen, J. B., Hansen, C. A., Kjems, J., Tommerup, N., Ruzzo, W. L. & Gorodkin, J. (2008). Comparative genomics beyond sequence-based alignments: RNA structures in the ENCODE regions. Genome Research, 18(2), 242-251. https://doi.org/10.1101/gr.6887408

Stissing, M., Mailund, T., Pedersen, C. S., Brodal, G. S. & Fagerberg, R. (2008). Computing the all-pairs quartet distance on a set of evolutionary trees. Journal of Bioinformatics and Computational Biology, 6(1), 37-50.

Chen, M. (2008). Conjugation of Reporter Groups and Aryltriazenes to Peptides and Oligonucleotides. Aarhus Universitetsforlag.

Chen, M. (2008). Conjugation of Reporter Groups and Aryltriazenes to Peptides and Oligonucleotides.

Fibiger, T. B. & Nielsen, K. H. (2008). Danske videnskabelige ekspeditioner: Forord. Jyske Historiker, (119).

Fibiger, T. B., Nielsen, K. H. & Petersen, R. H. (Eds.) (2008). Danske videnskabelige ekspeditioner: Den Jyske Historiker. (119 ed.) Den jyske historiker.

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