Single Protein-Mediated Vesicle Division

Publications

Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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De Putte, D. V., Meshaka, R., Trahin, B., Habart, E., Peeters, E., Berné, O., Alarcón, F., Canin, A., Chown, R., Schroetter, I., Sidhu, A., Boersma, C., Bron, E., Dartois, E., Goicoechea, J. R., Gordon, K. D., Onaka, T., Tielens, A. G. G. M., Verstraete, L. ... Zhen, J. (2024). PDRs4All VIII. Mid-infrared emission line inventory of the Orion Bar. Astronomy and Astrophysics, 687, Article A86. https://doi.org/10.1051/0004-6361/202449295

Eriksen, C. B. (2024). Peering Horizontally Through the Microscope: Stephen Gaukroger Explains the Middling World. In C. Wolfe & A. Waldow (Eds.), Science and the Shaping of Modernity: Essays in Honor of Stephen Gaukroger (pp. 113-121). Springer. https://doi.org/10.1007/978-3-031-76037-2_12

Hobolth, A., Rivas-González, I., Bladt, M. & Futschik, A. (2024). Phase-type distributions in mathematical population genetics: An emerging framework. Theoretical Population Biology, 157, 14-32. https://doi.org/10.1016/j.tpb.2024.03.001

Zhang, Y., Su, H., Wang, W., Dong, M. & Han, X. (2024). Phospholipid Epitaxial Assembly Behavior on a Hydrophobic Highly Ordered Pyrolytic Graphite Surface. Langmuir, 40(2), 1439-1446. https://doi.org/10.1021/acs.langmuir.3c03145

Teodori, L., Kroer, S., Omer, M., Andersen, V. L., Bech, P., Su, J., Bridoux, J., Nielsen, J. S., Bertelsen, M. B., Hernot, S., Gothelf, K. V. & Kjems, J. (2024). Plug-and-play nucleic acid-mediated multimerization of biparatopic nanobodies for molecular imaging. Molecular Therapy - Nucleic Acids, 35(3), Article 102305. https://doi.org/10.1016/j.omtn.2024.102305

Brodszkij, E., Ryberg, C., Lyons, J. A., Juhl, D. W., Nielsen, N. C., Sigalas, N. I., Lyulin, A. V., Pedersen, J. S. & Städler, B. (2024). Poly(Sitosterol)-Based Hydrophobic Blocks in Amphiphilic Block Copolymers for the Assembly of Hybrid Vesicles. Small, 20(40), Article 2401934. https://doi.org/10.1002/smll.202401934

Greussing, E., Guenther, L., Baram-Tsabari, A., Dabran-Zivan, S., Jonas, E., Klein-Avraham, I., Taddicken, M., Beets, B., Brossard, D., Chakraborty, A., Agergaard, T. E., Fage-Butler, A., Ju Huang, C., Nielsen, K. H., Kankaria, S., Lo, Y.-Y., Riedlinger, M. & Song, H. J. (2024). Predicting and describing the use of generative AI in science-related information search: Insights from a multinational survey. Abstract from Annual Conference of the “Science Communication” Division of the German Communication Association 2024, Zürich, Switzerland. https://www.ikmz.uzh.ch/dam/jcr:f29f21aa-1025-4b39-b848-4aefbd13a781/AI&SCICOMM24_Book%20of%20Abstracts.pdf

Nielsen, K. H. (2024). Problemet med Central Park. Weekendavisen, Sektion 4 (Ideer), 5.

Nielsen, K. H. (2024). Professorens prutter. Weekendavisen, Sektion 4 (Ideer), 5.

Malle, M. G., Song, P., Löffler, P. M. G., Kalisi, N., Yan, Y., Valero, J., Vogel, S. & Kjems, J. (2024). Programmable RNA Loading of Extracellular Vesicles with Toehold-Release Purification. Journal of the American Chemical Society, 146(18), 12410-12422. https://doi.org/10.1021/JACS.3C13123

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