Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

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Montasell, M. C., Raeven, L. M. V., Ravn Malm, M., van Hest, J. C. M. & Zelikin, A. N. (2025). Activation of enzymatic catalysis via nucleic acid hybridization affords synergistic coupling of specificity, potency, and signal amplification. Nature Communications, 16(1), Article 9638. https://doi.org/10.1038/s41467-025-64636-z

Elkhashab, M., Barreto, G., Fauconnier, M., Le Bourlout, Y., Creemers, L. B., Nieminen, H. J. & Howard, K. A. (2025). ADAMTS5-specific gapmer release from an albumin biomolecular assembly and cartilage internalization triggered by ultrasound. Drug Delivery, 32(1), Article 2464921. https://doi.org/10.1080/10717544.2025.2464921

Lund, A., Dorn, B., Jakob, T., Christensen, L. H., Jabs, F. & Spillner, E. (2025). A Fully Human Monoclonal Antibody Isolated From a Beekeeper Targets the Immunodominant IgE Epitope of Api m 10. Journal of Investigational Allergology and Clinical Immunology, 35(1), 55-57. https://doi.org/10.18176/jiaci.1029

Hobolth, A., Boitard, S., Futschik, A. & Leblois, R. (2025). A matrix-analytical sampling formula for time-homogeneous coalescent processes under the infinite sites mutation model. Theoretical Population Biology, 163, 62-79. https://doi.org/10.1016/j.tpb.2025.03.002

Tang, Z., Doktor, F. G., Jaganathan, R., Palotás, J., Oomens, J., Hornekær, L. & Hammer, B. (2025). Anharmonic infrared spectra of cationic pyrene and superhydrogenated derivatives. Journal of Chemical Physics, 163(4), Article 044304. https://doi.org/10.1063/5.0276133

Anthi, A. K., Kolderup, A., Vaage, E. B., Bern, M., Benjakul, S., Tjärnhage, E., Ruso-Julve, F., Jensen, K. R., Lode, H. E., Vaysburd, M., Nilsen, J., Herigstad, M. L., Sakya, S. A., Tietze, L., Pilati, D., Nyquist-Andersen, M., Dürkoop, M., Gjølberg, T. T., Peng, L. ... Andersen, J. T. (2025). An intranasal subunit vaccine induces protective systemic and mucosal antibody immunity against respiratory viruses in mouse models. Nature Communications, 16(1), Article 3999. Advance online publication. https://doi.org/10.1038/s41467-025-59353-6

Nielsen, K. H. (2025). Anonyme tip om snyd. Weekendavisen, Sektion 4 (Ideer), 5.

Søkilde, R., Kaadt, E., Kristensen, L. S., Venø, M. T., Kjems, J., Nyengaard, J. R., Nielsen, B. S. & Elfving, B. (2025). Application of microRNA In Situ Hybridization on Long-term Stored Human Formalin-fixed Paraffin-embedded Brain Samples from Psychiatric Patients. Molecular Neurobiology, 62(10), 12736-12746. https://doi.org/10.1007/s12035-025-05077-z

Kriebisch, C. M. E., Bantysh, O., Baranda Pellejero, L., Belluati, A., Bertosin, E., Dai, K., de Roy, M., Fu, H., Galvanetto, N., Gibbs, J. M., Gomez, S. S., Granatelli, G., Griffo, A., Guix, M., Gurdap, C. O., Harth-Kitzerow, J., Haugerud, I. S., Häfner, G., Jaiswal, P. ... Boekhoven, J. (2025). A roadmap toward the synthesis of life. Chem, 11(3), Article 102399. https://doi.org/10.1016/j.chempr.2024.102399

Makhaik, S., Rut, W., Choudhary, S., Upadhyay, T., Hao, C., Westberg, M., Bobst, C., Yoo, E., Fejzo, J., Lin, M. Z., Bogyo, M., Thompson, P., Drag, M. & Hardy, J. A. (2025). A robust fluorogenic substrate for chikungunya virus protease (nsP2) activity. Protein Science, 34(3), Article e70069. https://doi.org/10.1002/pro.70069

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