Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Howard, K. (2021). Biomolecular Assemblies as Multifunctional Drug Designs. In Handbook of Harnessing Biomaterials in Nanomedicine: Preparation, Toxicity, and Applications (2 ed., pp. 363-380). Jenny Stanford Publishing.

Noji, M., Samejima, T., Yamaguchi, K., So, M., Yuzu, K., Chatani, E., Akazawa-Ogawa, Y., Hagihara, Y., Kawata, Y., Ikenaka, K., Mochizuki, H., Kardos, J., Otzen, D. E., Bellotti, V., Buchner, J. & Goto, Y. (2021). Breakdown of supersaturation barrier links protein folding to amyloid formation. Communications Biology, 4(1), Article 120. https://doi.org/10.1038/s42003-020-01641-6

Poulsen, R. E., Scholz, L. A., Constantin, L., Favre-Bulle, I., Vanwalleghem, G. C. & Scott, E. K. (2021). Broad frequency sensitivity and complex neural coding in the larval zebrafish auditory system. Current Biology, 31(9), 1977-1987.e4. https://doi.org/10.1016/j.cub.2021.01.103

Zelikin, A. N. & Stellacci, F. (2021). Broad-Spectrum Antiviral Agents Based on Multivalent Inhibitors of Viral Infectivity. Advanced Healthcare Materials, 10(6), Article 2001433. https://doi.org/10.1002/adhm.202001433

Vanwalleghem, G., Constantin, L. & Scott, E. K. (2021). Calcium Imaging and the Curse of Negativity. Frontiers in Neural Circuits, 14, Article 607391. https://doi.org/10.3389/fncir.2020.607391

Nielsen, K. H. & Nielsen, H. (2021). Camp Century: The Untold Story of America's Secret Arctic Military Base Under the Greenland Ice. Colombia University Press.

Nielsen, K. H. & Nielsen, H. (2021, Jun 17). Camp Century in a Changing Arctic. Columbia University Press. https://www.cupblog.org/2021/06/17/camp-century-in-a-changing-arctic-by-kristian-h-nielsen-and-henry-nielsen/

Platts-Mills, T., Hilger, C., Jappe, U., van Hage, M., Gadermaier, G., Spillner, E., Lidholm, J., Aalberse, R. C., van Ree, R., Goodman, R. & Pomes, A. (2021). Carbohydrate Epitopes Currently Recognized as Targets for IgE Antibodies. Allergy: European Journal of Allergy and Clinical Immunology, 76(8), 2383-2394. https://doi.org/10.1111/all.14802.

Lassen, T. R., Just, J., Hjortbak, M. V., Jespersen, N. R., Stenz, K. T., Gu, T., Yan, Y., Su, J., Hansen, J., Bæk, R., Jørgensen, M. M., Nyengaard, J. R., Kristiansen, S. B., Drasbek, K. R., Kjems, J. & Bøtker, H. E. (2021). Cardioprotection by remote ischemic conditioning is transferable by plasma and mediated by extracellular vesicles. Basic Research in Cardiology, 116(1), Article 16. https://doi.org/10.1007/s00395-021-00856-w

Schutz, D., Conzelmann, C., Fois, G., Groß, R., Weil, T., Wettstein, L., Stenger, S., Zelikin, A., Hoffmann, T. K., Frick, M., Muller, J. A. & Munch, J. (2021). Carrageenan-containing over-the-counter nasal and oral sprays inhibit SARS-CoV-2 infection of airway epithelial cultures. American Journal of Physiology - Lung Cellular and Molecular Physiology, 320(5), L750-L756. https://doi.org/10.1152/ajplung.00552.2020

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