Single Protein-Mediated Vesicle Division

Publications

Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

Sort by: Date | Author | Title

Knudsen, H., Nielsen, H. & Nielsen, K. H. (2008). From private philanthropy to public funding. In H. Kragh, P. C. Kjærgaard, H. Nielsen & K. Hvidtfelt Nielsen (Eds.), Science in Denmark: A Thousand-Year History (1 ed., pp. 397-421). Aarhus Universitetsforlag.

Basse-O'Connor, A. (2008). Gaussian moving averages and semimartingales. Electronic Journal of Probability, 13, 1140-1165.

Sitte, J., Finster, K. & Küsel, K. (2008). Heavy metal tolerant sulfate-reducing prokaryotes in a gleyic creek soil from the former uranium mining district Ronneburg. Poster session presented at ISME conference 2008, Cairns, Australia.

Hobolth, A., Uyenoyama, M. K. & Wiuf, C. (2008). Importance sampling for the infinite sites model. Statistical Applications in Genetics and Molecular Biology, 7(1). https://doi.org/10.2202/1544-6115.1400

Xu, W., Dong, M., Gersen, H., Rauls, E., Vázquez-Campos, S., Crego-Calama, M., Reinhoudt, D. N., Laegsgaard, E., Stensgaard, I., Linderoth, T. R. & Besenbacher, F. (2008). Influence of alkyl side chains on hydrogen-bonded molecular surface nanostructures. Small, 4(10), 1620-3. https://doi.org/10.1002/smll.200800377

Sehgal, P., Sharma, D., Larsen, K. L., Wimmer, R., Otzen, D. & Doe, H. (2008). Influence of beta-Cyclodextrin on the Mixed Micellization Process of Sodium Dodecyl Sulfate and Sodium Lauroyl Sarcosine and Formation of Inclusion Complexes. Journal of Dispersion Science and Technology, 29, 128-133.

Sehgal, P., Sharma, M., Larsen, K. L., Wimmer, R., Doe, H. & Otzen, D. (2008). Interactions of g-Cyclodextrin with the Mixed Micelles of Anionic Surfactants and Their Inclusion Complexes Formation. Journal of Dispersion Science and Technology, 29, 885-890. https://doi.org/10.1007/s00396-006-1466-y

Sehgal, P., Sharma, M., Larsen, K. L., Wimmer, R., Doe, H. & Otzen, D. (2008). Interactions of γ-Cyclodextrin with the Mixed Micelles of Anionic Surfactants and Their Inclusion Complexes Formation. Journal of Dispersion Science and Technology, 29, 885-890. https://doi.org/10.1080/01932690701783390

Rahbek, U. L., Howard, K. A., Oupicky, D., Manickam, D. S., Dong, M., Nielsen, A. F., Hansen, T. B., Besenbacher, F. & Kjems, J. (2008). Intracellular siRNA and precursor miRNA trafficking using bioresponsive copolypeptides. Journal of Gene Medicine, 10(1), 81-93. https://doi.org/10.1002/jgm.1120

Nygaard, J. V., Andersen, M. Ø., Howard, K. A., Foss, M., Bünger, C., Kjems, J. & Besenbacher, F. (2008). Investigation of particle-functionalized tissue engineering scaffolds using X-ray tomographic microscopy. Biotechnology and Bioengineering (Print), 100(4), 820-9. https://doi.org/10.1002/bit.21796

Displaying results 3011 to 3020 out of 3469

302