Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Schiølin, K. H. (2016). Cum Deus Calculat, Fit Mundus, or The Will to Technology: Diagnosis and Cure. A Peer-Reviewed Journal About, 5(1). http://www.aprja.net/?p=3083

Bortolini, C. & Dong, M. (2016). Cystine oligomers successfully attached to peptide cysteine-rich fibrils. Frontiers of Chemical Science and Engineering, 10(1), 99-102. https://doi.org/10.1007/s11705-016-1554-6

Hall, J. P., Keane, P. M., Beer, H., Buchner, K., Winter, G., Sorensen, T. L., Cardin, D. J., Brazier, J. A. & Cardin, C. J. (2016). Delta chirality ruthenium 'light-switch' complexes can bind in the minor groove of DNA with five different binding modes. Nucleic Acids Research, 44(19), 9472-9482. https://doi.org/10.1093/nar/gkw753

Kim, J.-Y., Sahu, S., Yau, Y.-H., Wang, X., Shochat, S. G., Nielsen, P. H., Dueholm, M. S., Otzen, D. E., Lee, J., Delos Santos, M. M. S., Yam, J. K. H., Kang, N.-Y., Park, S.-J., Kwon, H., Seviour, T. W., Yang, L., Givskov, M. & Chang, Y.-T. (2016). Detection of pathogenic biofilms with bacterial amyloid targeting fluorescent probe, CDy11. Journal of the American Chemical Society, 138(1), 402-407. https://doi.org/10.1021/jacs.5b11357

Jordal, P. L., Dyrlund, T. F., Winge, K., Larsen, M. R., Danielsen, E. H., Wells, J. A., Otzen, D. E. & Enghild, J. J. (2016). Detection of proteolytic signatures for Parkinson's disease. Future Neurology, 11(1), 15-32. https://doi.org/10.2217/fnl.16.3

Wang, J., Zhu, Z., Bortolini, C., Hoffmann, S. V., Amari, A., Zhang, H. X., Liu, L. & Dong, M. D. (2016). Dimensionality of carbon nanomaterial impacting on the modulation of amyloid peptide assembly. Nanotechnology, 27(30), Article 304001. https://doi.org/10.1088/0957-4484/27/30/304001

Nielsen, K. H. (2016). Diskurs. Weekendavisen, Ideer, 10.

Nielsen, K. H. (2016). Dna. Weekendavisen, Ideer, 10.

Okholm, A. H. & Kjems, J. (2016). DNA nanovehicles and the biological barriers. Advanced Drug Delivery Reviews, 106(Part A), 183-191. Article 16. https://doi.org/10.1016/j.addr.2016.05.024

Kodal, A. L. B., Rosen, C. B., Mortensen, M. R., Tørring, T. & Gothelf, K. V. (2016). DNA-Templated Introduction of an Aldehyde Handle in Proteins. ChemBioChem, 17(14), 1338-1342. https://doi.org/10.1002/cbic.201600254

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