Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Nielsen, K. H. (2010). Sixth Framework Programme. In D. H. Guston (Ed.), Encyclopedia of Nanotechnology and Society (pp. 717-718). SAGE Publications.

Nielsen, K. H. & Thomasen, L. S. (2010). Smilla, Anna og Alexandra: Videnskabskvinder i nyere dansk populærlitteratur. Slagmark: tidsskrift for idéhistorie , 58, 85-98.

Knudsen, M., Feliu, E., Wiuf, C. H. & Andersen, L. N. (2010). Steady States of Phosphorelays and Two-Component Systems. Poster session presented at The 11th International Conference on Systems Biology, Edinburgh, United Kingdom.

Binder, M., Léchenne, B., Ummanni, R., Balabanov, S., Trepel, M., Scharf, C., Trusch, M., Schlüter, H., Braren, I. & Spillner, E. (2010). Stereotypical chronic lymphocytic leukemia B-cell receptors recognize survival promoting antigens on stromal cells. PLoS One, 5(12), Article e15992. https://doi.org/10.1371/journal.pone.0015992

Ferkinghoff-Borg, J., Fonslet, J., Andersen, C. B., Krishna, S., Pigolotti, S., Yagi, H., Goto, Y., Otzen, D. & Jensen, M. H. (2010). Stop-and-go kinetics in amyloid fibrillation. Physical Review E. Statistical, Nonlinear, and Soft Matter Physics, 82(1 Pt 1), 010901.

Giehm, L. & Otzen, D. E. (2010). Strategies to increase the reproducibility of protein fibrillization in plate reader assays. Analytical Biochemistry, 400(2), 270-81. https://doi.org/10.1016/j.ab.2010.02.001

Giehm, L. & Otzen, D. (2010). Strategies to increase the reproducibility of α-synuclein fibrillation in plate reader assays. Analytical Biochemistry, 406(2), 270-281.

Spillner, E., Laursen, N. S., Christensen, J. B., Jensen, M., Fredslund, F., Sottrup-Jensen, L., Andersen, G. R., Gordon, N., Hermans, S., Lorenz, N., Jackson, N., Fraser, J. D., Wines, B. & Bjerre, M. (2010). Structural basis for inhibition of complement C5 by the SSL7 protein from Staphylococcus aureus. Proceedings of the National Academy of Sciences (PNAS), 107(8), 3681-3686. https://doi.org/10.1073/pnas.0910565107

Hobolth, A. & Jensen, J. L. (2010). Summary statistics for end-point conditioned continuous-time Markov chains. Thiele Centre, Institut for Matematiske Fag, Aarhus Universitet.

Xu, W., Wang, J.-G., Jacobsen, M. F., Mura, M., Yu, M., Kelly, R. E. A., Meng, Q.-Q., Lægsgaard, E., Stensgaard, I., Linderoth, T. R., Kjems, J., Kantorovich, L. N., Gothelf, K. V. & Besenbacher, F. (2010). Supramolecular porous network formed by molecular recognition between chemically modified nucleobases guanine and cytosine. Angewandte Chemie International Edition, 49(49), 9373-7. https://doi.org/10.1002/anie.201003390

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