Single Protein-Mediated Vesicle Division

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Recent research news on Artificial Biology

The successful reconstitution of Drs2p–Cdc50p flippase in small and giant polymer lipid hybrid vesicles is illustrated. The hybrid vesicles show adenosine 5′-triphosphate consumption and flipping of 2-dioleoyl-sn-glycero-3-phospho-l-serine lipids from the inner to the outer leaflet. This induced transmembrane asymmetry results in constriction and division of the giant hybrid vesicles.

4 September 2025 by Emilia Djomina Hansen

A collaborative study between the Lyons and Stadler labs, published in Advanced Materials, reports the successful reconstitution of the Drs2p–Cdc50p lipid flippase in polymer–lipid hybrid vesicles, enabling active translocation of negatively charged lipids to establish transmembrane asymmetry. Crucially, the chemical nature of the hydrophobic block in the amphiphilic block copolymers determines the curvature changes that drive vesicle constriction and division. This work marks an important step toward constructing synthetic systems capable of protein-mediated division, advancing the goal of bottom-up self-replicating assemblies.

Read the article published in Advanced Marterials here.

Contact information:
Professor Brigitte Maria Städler
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: bstadler@inano.au.dk

Tenure track Assistant Professor Joseph Lyons
Aarhus University
Interdisciplinary Nanoscience Center (iNANO)
Email: lyons@inano.au.dk

Recent publications by network

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Finster, K., Thomsen, T. R. & Ramsing, N. B. (2001). Desulfomusa hansenii gen.nov., sp.nov., a novel marine propionate-degrading, sulfate-reducing bacterium isolated from Zostera marina roots. International Journal of Systematic and Evolutionary Microbiology, 51, 2055-2061.

Andersen, J. P., Sørensen, T. L.-M., Povlsen, K. & Vilsen, B. (2001). Importance of transmembrane segment M3 of the sarcoplasmic reticulum Ca²⁺-ATPase for control of the gateway to the Ca²⁺-sites. Journal of Biological Chemistry, 276, 23312-23321.

Dirac, A. G. & Kjems, J. (2001). Mapping DNA sites of HIV-1 integrase by protein footprinting. FEBS journal, 268, 743-751.

Skovhus, T. L. & Finster, K. (2001). Når bakterier snakker. Aktuel Naturvidenskab, 2, 4-7.

Verhoef, K., Bilodeau, P. S., nullWamelJ.L.B., V., Kjems, J., Stoltzfus, C. M. & Berkhout, B. (2001). Repair of a Rev-minus HIV-1 mutant by activation of a cryptic splice site. J. Virol., 75, 3495-3500.

de Wit, R., Stal, L. J., Lomstein, B. A., Herbert, R. A., van Gemerden, H., Viaroli, P., Cecherelli, V.-U., Rodrigues-Valera, F., Bartoli, M., Giordani, G., Azzoni, R., Schaub, B., Welsh, T. D., Donelly, A., Cifuentes, A., Antón, J., Finster, K., Nielsen, L. B., Pedersen, A.-GU. ... Heijs, S. K. (2001). ROBUST: The ROle of BUffering capacities in STabilising Coastal lagoon ecosystems. Continental Shelf Research, 21, 2021-2041. http://www.elsevier.com/locate/csr

Tange, T. Ø. & Kjems, J. (2001). SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain. J. Mol. Biol., 312, 649-662.

Hornekær, L., Kjærggard, N., Thommesen, A. M. & Drewsen, M. (2001). Structural properties of two-component Coulomb crystals in linear Paul traps. Physical Review Letters, 86, 1994.

Nielsen, L. B., Finster, K., Welsh, D. T., Donelly, A., Herbert, R. A., de Wit, R. & Lomstein, B. A. (2001). Sulphate reduction and nitrogen fixation rates associated with roots, rhizomes and sediments from Zostera noltii and Spartina maritima meadows. Environmental Microbiology, 3(1), 63-71.

Brodal, G. S., Fagerberg, R., Pedersen, C. N. S. & Östlin, A. (2001). The Complexity of Constructing Evolutionary Trees Using Experiments. In F. Orejas, P. G. Spirakis & J. van Leeuwen (Eds.), Automata, Languages and Programming: 28th International Colloquium, ICALP 2001 Crete, Greece, July 8–12, 2001 Proceedings (pp. 140-151). Springer. https://doi.org/10.1007/3-540-48224-5_12

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