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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Ebbesen, K. K., Hansen, T. B. & Kjems, J. (2017). Insights into circular RNA biology. RNA Biology, 14(8), 1035-1045. https://doi.org/10.1080/15476286.2016.1271524

Qian, X., Lin, Z., Guan, L., Li, Q., Wang, Y., Zhang, M. & Dong, M. (2017). In Situ Probing the Relaxation Properties of Ultrathin Polystyrene Films by Using Electric Force Microscopy. Nanoscale Research Letters, 12, Article 257. https://doi.org/10.1186/s11671-017-2019-7

Kristoffersen, E. L., Jørgensen, A. G., Jørgensen, L. A., Jensen, P. W., W Byl, J. A., Osheroff, N., Andersen, A. H., Stougaard, M., Ho, Y.-P. & Knudsen, B. R. (2017). Interlinked DNA nano-circles for measuring topoisomerase II activity at the level of single decatenation events. Nucleic Acids Research, 45(13), 7855-7869. https://doi.org/10.1093/nar/gkx480

Fay, F., Hansen, L., Hectors, S. J. C. G., Sanchez-Gaytan, B. L., Zhao, Y., Tang, B.-J., Munitz, J., Alaarg, A., Braza, M. S., Gianella, A., Aaronson, S. A., Reiner, T., Kjems, J., Langer, R., Hoeben, F. J. M., Janssen, H. M., Calcagno, C., Strijkers, G. J., Fayad, Z. A. ... Mulder, W. J. M. (2017). Investigating the Cellular Specificity in Tumors of a Surface-Converting Nanoparticle by Multimodal Imaging. Bioconjugate Chemistry, 28(5), 1413-1421. https://doi.org/10.1021/acs.bioconjchem.7b00086

Nielsen, K. H. (2017). Italesættelse. Weekendavisen, Sektion 4 (Ideer), 10.

Nielsen, K. H. (2017). Kapitalocæn. Weekendavisen, Sektion 4 (Ideer), 10.

Nielsen, K. H. (2017). Kemoterapi. Weekendavisen, Sektion 4 (Ideer), 10.

Yang, G., Liu, L., Wang, J., Bortolini, C. & Dong, M. (2017). Light-driven porphyrin modulating fibrillation of hIAPP(20-29) peptide. Journal of Colloid and Interface Science, 495, 37-43. https://doi.org/10.1016/j.jcis.2017.01.089

Li, Y., Slemming-Adamsen, P., Wang, J., Song, J., Wang, X., Yu, Y., Dong, MD., Chen, C., Besenbacher, F. & Chen, M. (2017). Light responsive hybrid nanofibres for on-demandtherapeutic drug and cell delivery. Journal of Tissue Engineering and Regenerative Medicine, 11(8), 2411–2420. https://doi.org/10.1002/term.2169

Mazur, F., Bally, M., Stadler, B. M. & Chandrawati, R. (2017). Liposomes and lipid bilayers in biosensors. Advances in Colloid and Interface Science, 249, 88-99. https://doi.org/10.1016/j.cis.2017.05.020

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