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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Quinones, J. P., Gothelf, K. V., Kjems, J., Heras, A., Schmidt, C. & Peniche, C. (2013). Novel Self-Assembled Nanoparticles of Testosterone-Modified Glycol Chitosan and Fructose Chitosan for Controlled Release. Journal of Biomaterials and Tissue Engineering, 3(1), 164-172. https://doi.org/10.1166/jbt.2013.1071

Balakrishnan, V. S., Vad, B. S. & Otzen, D. (2013). Novicidin's membrane permeabilizing activity is driven by membrane partitioning but not by helicity: A biophysical study of the impact of lipid charge and cholesterol. BBA General Subjects, 1834(6), 996-1002. https://doi.org/10.1016/j.bbapap.2013.03.025

Schiølin, K. H. & Riis, S. (Eds.) (2013). Nye spørgsmål om teknikken. Aarhus Universitetsforlag.

Deva, T., Lorenzen, N., Vad, B. S., Petersen, S. V., Thørgersen, I., Enghild, J. J., Kristensen, T. & Otzen, D. (2013). Off-pathway aggregation can inhibit fibrillation at high protein concentrations. BBA General Subjects, 1834(3), 677-687. https://doi.org/10.1016/j.bbapap.2012.12.020

Wang, H., Andersen, K. K., Vad, B. S. & Otzen, D. E. (2013). OmpA can form folded and unfolded oligomers. Biochimica et Biophysica Acta - Proteins and Proteomics, 1834(1), 127-136. https://doi.org/10.1016/j.bbapap.2012.09.002

Basse-O'Connor, A. & Rosiński, J. (2013). On Lévy’s Equivalence Theorem in Skorohod space. In C. Houdré, D. M. Mason, J. Rosi´nski & J. A. Wellner (Eds.), High dimensional probability VI: The Banff volume (pp. 219-225). Birkhäuser Verlag. https://doi.org/10.1007/978-3-0348-0490-5_14

Basse-O'Connor, A. & Rosinski, J. (2013). On the uniform convergence of random series in Skorohod space and representations of càdlàg infinitely divisible processes. Annals of Probability, 41(6), 4317-4341. https://doi.org/10.1214/12-AOP783

Li, J., Han, Q., Zhang, Y., Yang, R., Wang, C., Zhang, W., Dong, M. & Besenbacher, F. (2013). Optical regulation of protein adsorption and cell adhesion by photoresponsive GaN nanowires. A C S Applied Materials and Interfaces, 5(19), 9816-9822. https://doi.org/10.1021/am403070g

Antaloae, A. V., Montigny, C., le Maire, M., Watson, K. A. & Sørensen, T. L.-M. (2013). Optimisation of recombinant production of active human cardiac SERCA2a ATPase. PLoS One, 8(8), e71842. https://doi.org/10.1371/journal.pone.0071842

Iversen, F., Yang, C., Dagnæs-Hansen, F., Schaffert, D. H., Kjems, J. & Gao, S. (2013). Optimized siRNA-PEG Conjugates for Extended Blood Circulation and Reduced Urine Excretion in Mice. Theranostics, 3(3), 201-209. https://doi.org/10.7150/thno.5743

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Kalinka Hansen