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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Jakob, T., Rauber, M. M., Perez-Riverol, A., Spillner, E. & Blank, S. (2020). The Honeybee Venom Major Allergen Api m 10 (Icarapin) and Its Role in Diagnostics and Treatment of Hymenoptera Venom Allergy. Current Allergy and Asthma Reports, 20(9), Article 48. https://doi.org/10.1007/s11882-020-00943-3
van Gils, J. H. M., van Dijk, E., Peduzzo, A., Hofmann, A., Vettore, N., Schützmann, M. P., Groth, G., Mouhib, H., Otzen, D. E., Buell, A. K. & Abeln, S. (2020). The hydrophobic effect characterises the thermodynamic signature of amyloid fibril growth. PLoS Computational Biology, 16(5), Article e1007767. https://doi.org/10.1371/journal.pcbi.1007767
van Diggelen, F., Frank, S. A., Somavarapu, A. K., Scavenius, C., Apetri, M. M., Nielsen, J., Tepper, A. W. J. W., Enghild, J. J. & Otzen, D. E. (2020). The interactome of stabilized α-synuclein oligomers and neuronal proteins. FEBS journal, 287(10), 2037-2054. https://doi.org/10.1111/febs.15124
Marvian, A. T., Aliakbari, F., Mohammad-Beigi, H., Ahmadi, Z. A., Mehrpouyan, S., Lermyte, F., Nasouti, M., Collingwood, J. F., Otzen, D. E. & Morshedi, D. (2020). The status of the terminal regions of α-synuclein in different forms of aggregates during fibrillization. International Journal of Biological Macromolecules, 155, 543-550. https://doi.org/10.1016/j.ijbiomac.2020.03.238
Su, Y., Zhang, E., Wang, Y., Li, Q., Chen, M. & Dong, MD. (2020). Tunable Hierarchical Wrinkling Surface via Microscale Patterned Vertical Deformation. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 607, Article 125468. https://doi.org/10.1016/j.colsurfa.2020.125468
Uhd, J., Miotke, L., Ji, H. P., Dunaeva, M., Pruijn, G. J. M., Jørgensen, C. D., Kristoffersen, E. L., Birkedal, V., Yde, C. W., Nielsen, F. C., Hansen, J. & Astakhova, K. (2020). Ultra-fast detection and quantification of nucleic acids by amplification-free fluorescence assay. Analyst, 145(17), 5836-5844. https://doi.org/10.1039/d0an00676a
Kang, S., He, M., Chen, M., Wang, J., Zheng, L., Chang, X., Duan, H., Sun, D., Dong, M. & Cui, L. (2020). Ultrafast plasma immersion strategy for rational modulation of oxygen-containing and amino groups in graphitic carbon nitride. Carbon, 159, 51-64. https://doi.org/10.1016/j.carbon.2019.12.022
Liu, Y., He, M., Guo, R., Fang, Z., Kang, S., Ma, Z., Dong, M., Wang, W. & Cui, L. (2020). Ultrastable metal-free near-infrared-driven photocatalysts for H2 production based on protonated 2D g-C3N4 sensitized with Chlorin e6. Applied Catalysis B: Environment and Energy, 260, Article 118137. https://doi.org/10.1016/j.apcatb.2019.118137
Rasmussen, H. Ø., Enghild, J. J., Otzen, D. E. & Pedersen, J. S. (2020). Unfolding and partial refolding of a cellulase from the SDS-denatured state: From β-sheet to α-helix and back. B B A - General Subjects, 1864(1), 129434 1-12. Article 129434. https://doi.org/10.1016/j.bbagen.2019.129434
Zelikin, A. N. (2020). Unique enzymatic repertoire reveals the tumour. Nature Chemistry, 12, 11-12. https://doi.org/10.1038/s41557-019-0400-0

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Revised 03.03.2026
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Kalinka Hansen