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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Nielsen, K. H. (2005). Haciendo que las 'Cosas' ocurran: Recensión critica. Bruno Latour and Peter Weibel, Eds. Making Things Public. Atmospheres of Democracy. MIT Press. 2005. AIBR Revista de Antropología. http://www.plazamayor.net/antropologia/44nov/libros/nov0502.pdf

Furnes, C., Arnesen, T., Askjaer, P., Kjems, J. & Szilvay, A. M. (2005). HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain. Retrovirology, 2, 39. https://doi.org/10.1186/1742-4690-2-39

Bockisch, B., Grunwald, T., Spillner, E. & Bredehorst, R. (2005). Immobilized stem-loop structured probes as conformational switches for enzymatic detection of microbial 16S rRNA. Nucleic Acids Research, 33(11), Article e101. https://doi.org/10.1093/nar/gni101

Hornekær, L., Baurichter, A., Petrunin, V. V., Luntz, A. C., Kay, B. D. & Al-Halabi, A. (2005). Influence of surface morphology on D₂ desorption kinetics from Amorphous Solid Water. Journal of Chemical Physics, 122, 124701.

Mailund , Pedersen, C. N. S., Bardino, J., Vinter, B. & Karlsen, H. H. (2005). Initial experiences with GeneRecon on MiG. In Proceedings of The 2005 International Conference on Grid Computing and Applications (GCA)

Jensen, A. & Finster, K. (2005). Isolation and characterization of Sulfurospirillum carboxydovorans sp. nov., a new microaerophilic carbon monoxide oxidizing epsilon Proteobacterium. Antonie van Leeuwenhoek: Journal of Microbiology, 87, 339-353.

Kjems, J., Bünger, C., Nygaard, J. V. & Mygind, T. (2005). Making implants last a lifetime. In iNANO Annual Report 2005 (pp. 14-15).

Gorjanović, S., Beljanski, M. V., Pavlović, M., Spillner, E., Gorjanović, R. & Gojgić-Cvijanović, G. (2005). Malting barley grain non-specific lipid-transfer protein (ns-LTP): Importance for grain protection. Journal of the Institute of Brewing, 111(2), 99-104.

Niemann, H., Elvert, M., Hovland, M., Orcutt, B., Judd, A., Suck, I., Gutt, J., Joye, S., Damm, E., Finster, K. & Boetius, A. (2005). Methane emission and consumption at a North Sea gas seep (Tommeliten area). Biogeosciences, 2, 335-351.

Mogensen, J. E., Kleinschmidt, J. H., Schmidt, M. A. & Otzen, D. (2005). Misfolding of a bacterial autotransporter. Protein Science, 14, 2814-2827. https://doi.org/10.1110/ps.051628705

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