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Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Petersen, S., Kläning, E., Andersen, B., Cameron, J., Sørensen, E. S. & Howard, K. (2016). Neonatal Fc Receptor Binding Tolerance toward the Covalent Conjugation of Payloads to Cysteine 34 of Human Albumin Variants. Molecular Pharmaceutics, 13(2), 677–682. https://doi.org/10.1021/acs.molpharmaceut.5b00605

Jakobsen, H. J., Song, L., Gan, Z., Hung, I., Bildsoe, H., Skibsted, J., Bak, E. N., Finster, K., Nornberg, P. & Jensen, S. K. (2016). NMR and EPR Studies of Free-Radical Intermediates from Experiments Mimicking the Winds on Mars: A Sink for Methane and Other Gases. The Journal of Physical Chemistry Part C, 120(45), 26138-26149. https://doi.org/10.1021/acs.jpcc.6b08847

Nielsen, K. H. (2016). Nordic Perspectives on STS. Abstract from DASTS 2016, Aarhus, Denmark.

Nielsen, K. H. (2016). Nordic STS: A tentative historical, conceptual and geographical exploration. Abstract from Swedish STS Conference 2016, Helsingborg, Sweden.

Eriksen, C. B., Carstensen, J. & Meedom, P. J. (Eds.) (2016). Ny Jord - Tidsskrift for naturkritik: Nr. 2. Forlaget Virkelig.

Hirsch, C. (2016). On the absence of percolation in a line-segment based lilypond model. In Annales de l'Institut Henri Poincaré, Probabilités et Statistiques (Vol. 52, pp. 127-145)

Montigny, C., Lyons, J., Champeil, P., Nissen, P. & Lenoir, G. (2016). On the molecular mechanism of flippase- and scramblase-mediated phospholipid transport. B B A - Molecular and Cell Biology of Lipids, 1861(8, Part B), 767-783. https://doi.org/10.1016/j.bbalip.2015.12.020

Sahyoun, M., Wex, H., Gosewinkel, U. B., Santl-Temkiv, T., Nielsen, N. W., Finster, K., Sørensen, J. H., Stratmann, F. & Korsholm, U. S. (2016). On the usage of classical nucleation theory in quantification of the impact of bacterial INP on weather and climate. Atmospheric Environment, 139, 230-240. https://doi.org/10.1016/j.atmosenv.2016.05.034

Basse-O'Connor, A. & Weber, M. (2016). On the Φ-variation of stochastic processes with exponential moments. Transactions of the London Mathematical Society, 3(1), 1-27. https://doi.org/10.1112/tlms/tlw001

Nielsen, K. H. (2016). Open access. Weekendavisen, Ideer, 10.

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