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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Eriksen, C. B. (2017). Review of: Baker and Gibbs (eds.): Life Forms in the Thinking of the Long Eighteenth Century. Isis, 108(4), 907-909.

Weizmann, Y. & Andersen, E. S. (2017). RNA nanotechnology-The knots and folds of RNA nanoparticle engineering. MRS Bulletin, 42(12), 930-935. https://doi.org/10.1557/mrs.2017.277

Mooney, C. M., Jimenez-Mateos, E. M., Engel, T., Mooney, C., Diviney, M., Venø, M. T., Kjems, J., Farrell, M. A., O'Brien, D. F., Delanty, N. & Henshall, D. C. (2017). RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis. Scientific Reports, 7, Article 41517. https://doi.org/10.1038/srep41517

Nielsen, K. H. (2017). Robot. Weekendavisen, Sektion 4 (Ideer), 10.

Nielsen, K. H. (2017, Dec 30). Sådan blev raketterne opfundet. https://videnskab.dk/teknologi-innovation/saadan-blev-raketterne-opfundet

Lyons, J. A., Bøggild, A., Nissen, P. & Frauenfeld, J. (2017). Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters. In C. Ziegler (Ed.), Methods in Enzymology: A Structure-Function Toolbox for Membrane Transporter and Channels (Vol. 594, pp. 85-99). Academic Press. https://doi.org/10.1016/bs.mie.2017.06.035

Nielsen, K. H. (2017). Science popularization goes global: UNESCO’s Division of Science & Its Popularization. Abstract from 25th International Congress of History of Science and Technology, Rio de Janeiro, Brazil. http://www.ichst2017.sbhc.org.br/download/download?ID_DOWNLOAD=170

Camilli, L., Jorgensen, J. H., Tersoff, J., Stoot, A. C., Balog, R., Cassidy, A., Sadowski, J. T., Boggild, P. & Hornekaer, L. (2017). Self-assembly of ordered graphene nanodot arrays. Nature Communications, 8(1), Article 47. https://doi.org/10.1038/s41467-017-00042-4

Otzen, D. E., Vad, B. S., Dueholm, M. S., Nielsen, P. H., Rouse, S. L. & Matthews, S. J. (2017). Self-organizing amyloid in bacteria. European Biophysics Journal, 46, S98-S98.

Otzen, D. E., Vad, B. S., Dueholm, M. S., Nielsen, P. H., Rouse, S. L. & Matthews, S. J. (2017). Self-organizing amyloid in bacteria. European Biophysics Journal, 46(supp 1), S341-S341. https://doi.org/10.1007/s00249-017-1222-x

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