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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Frislev, H. S., Boye, T. L., Nylandsted, J. & Otzen, D. (2017). Liprotides kill cancer cells by disrupting the plasma membrane. Scientific Reports, 7(1), Article 15129. https://doi.org/10.1038/s41598-017-15003-6

Nærdal, I., Netzer, R., Irla, M., Krog, A., Heggeset, T. M. B., Wendisch, V. F. & Brautaset, T. (2017). L-lysine production by Bacillus methanolicus: Genome-based mutational analysis and L-lysine secretion engineering. Journal of Biotechnology, 244, 25-33. https://doi.org/10.1016/j.jbiotec.2017.02.001

Chandrawati, R., Chang, J. Y. H., Reina-Torres, E., Jumeaux, C., Sherwood, J. M., Stamer, W. D., Zelikin, A. N., Overby, D. R. & Stevens, M. M. (2017). Localized and Controlled Delivery of Nitric Oxide to the Conventional Outflow Pathway via Enzyme Biocatalysis: Toward Therapy for Glaucoma. Advanced Materials, 29(16), Article 1604932. https://doi.org/10.1002/adma.201604932

Schandock, F., Riber, C. F., Röcker, A., Müller, J. A., Harms, M., Gajda, P., Zuwala, K., Andersen, A. H. F., Løvschall, K. B., Tolstrup, M., Kreppel, F., Münch, J. & Zelikin, A. N. (2017). Macromolecular Antiviral Agents against Zika, Ebola, SARS, and Other Pathogenic Viruses. Advanced Healthcare Materials, 6(23), Article 1700748. https://doi.org/10.1002/adhm.201700748

Riber, C. F., Hinton, T. M., Gajda, P., Zuwala, K., Tolstrup, M., Stewart, C. & Zelikin, A. N. (2017). Macromolecular Prodrugs of Ribavirin: Structure-Function Correlation as Inhibitors of Influenza Infectivity. Molecular Pharmaceutics, 14(1), 234-241. https://doi.org/10.1021/acs.molpharmaceut.6b00826

Banerjee, C., Westberg, M., Breitenbach, T., Bregnhøj, M. & Ogilby, P. R. (2017). Monitoring Interfacial Lipid Oxidation in Oil-in-Water Emulsions Using Spatially Resolved Optical Techniques. Analytical Chemistry, 89(11), 6239-6247. https://doi.org/10.1021/acs.analchem.7b01228

Taipaleenmäki, E. M., Mouritzen, S., Schattling, P., Zhang, Y. & Stadler, B. M. (2017). Mucopenetrating Micelles with a PEG Corona. Nanoscale, 9(46), 18438-18448. https://doi.org/10.1039/C7NR06821B

Liu, Y., Zhou, G., Liu, Z., Guo, M., Jiang, X., Taskin, M. B., Zhang, Z., Liu, J., Tang, J., Bai, R., Besenbacher, F., Chen, M. & Chen, C. (2017). Mussel Inspired Polynorepinephrine Functionalized Electrospun Polycaprolactone Microfibers for Muscle Regeneration. Scientific Reports, 7(1), 8197. Article 8197. https://doi.org/10.1038/s41598-017-08572-z

Mortensen, H. G., Madsen, J. K., Andersen, K. K., Vosegaard, T., Deen, G. R., Otzen, D. E. & Pedersen, J. S. (2017). Myoglobin and α-Lactalbumin Form Smaller Complexes with the Biosurfactant Rhamnolipid Than with SDS. Biophysical Journal, 113(12), 2621-2633. https://doi.org/10.1016/j.bpj.2017.10.024

Chen, M. (2017). Nanofiber Technology for Three dimensional Nanobiointerfaces and Cellular Engineering. Abstract from 20th international conference on advanced nanotechnology, Amsterdam, Netherlands.

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Kalinka Hansen