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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Laursen, M. B., Pakula, M. M., Gao, S., Fluiter, K., Mook, O. R., Baas, F., Langklaer, N., Wengel, S. L., Wengel, J., Kjems, J. & Bramsen, J. B. (2010). Utilization of unlocked nucleic acid (UNA) to enhance siRNA performance in vitro and in vivo. Molecular BioSystems, 6(5), 862-70. https://doi.org/10.1039/b918869j

Nielsen, K. H. & Andersen, C. (2010). Vi har aldrig været politiske! Bruno Latours politiske filosofi og nonmodernitet. Slagmark: tidsskrift for idéhistorie , (59), 117-130.

Nielsen, K. H. (2010). We Have Never Been Scientists. Annals of Science, 67(4), 561-567. https://doi.org/10.1080/00033790902788055

Hubert, C., Loy, A., Nickel, M., Arnosti, C., Baranyi, C., Brüchert, V., Ferdelman, T., Finster, K., Christensen, F. M., Rezende, J. R. D., Vandieken, V. & Jørgensen, B. B. (2009). A constant flux of diverse thermophilic bacteria into the cold arctic seabed. Science, 325, 1541-1544. https://doi.org/10.1126/science.1174012

Sitte, J., Akob, D., Kaufmann, C., Pollok, K., Finster, K., Kostka, J. & Küsel, K. (2009). Activity and identification of sulfate reducing prokaryotes in heavy metal and radionuclide contaminated creek soil: Verein für allgemeine und angewandte Mikrobiologie. Poster session presented at VAAM conference 2009.

Sitte, J., Kaufmann, C., Pollok, K., Finster, K., Langenhorst, F. & Küsel, K. (2009). Activity of sulfate reducing prokaryotes in heavy metal and radionuclide contaminated creek soil at a former mining district. Poster session presented at FEMS conference 2009, Göteborg, Sweden.

Besenbacher, S., Pedersen, C. S. & Mailund, T. (2009). A fast algorithm for genome wide haplotype pattern mining. BMC Bioinformatics, (suppl. 1), 772. https://doi.org/10.1186/1471-2105-10-S1-S74

Gavardi, E., Cuppen, H. M. & Hornekær, L. (2009). A kinetic Monte Carlo study of desorption of H₂ from graphite (0001). Chemical Physics Letters, 477(4-6), 285–289. https://doi.org/10.1016/j.cplett.2009.07.003

Bramsen, J. B., Laursen, M. B., Nielsen, A. F., Hansen, T. B., Bus, C., Langkjær, N., Babu, B. R., Højland, T., Abramov, M., Van Aerschot, A., Odadzic, D., Smicius, R., Haas, J., Andree, C., Barman, J., Wenska, M., Srivastava, P., Zhou, C., Honcharenko, D. ... Kjems, J. (2009). A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity. Nucleic Acids Research, 37(9), 2867-81. https://doi.org/10.1093/nar/gkp106

Dutheil, J., Ganapathy, G., Hobolth, A., Mailund, T., Uyenoyama, M. & Schierup, M. H. (2009). Ancestral population genomics: the coalescent hidden Markov model approach. Genetics, 183(1), 259–274. https://doi.org/10.1534/genetics.109.103010

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