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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Pedersen, A. B., Andersen, D. G., Jakobsen, J. H., Montasell, M. C. & Zelikin, A. N. (2025). Receptor-Mediated Transmembrane Activation of Protein Folding in Synthetic Cells. Bioconjugate Chemistry, 36(4), 782-791. https://doi.org/10.1021/acs.bioconjchem.5c00043

Skovborg, G., Svejsø, F. H., Müller, C., Jensen, B. N., Jensen, J. G., Majidi, S. E., Matthiesen, C. L. & Chen, M. (2025). Replication of patient specific circulating tumor cells on a microfibrous filter for drug screening. Nanoscale, 17(18), 11592-11604. https://doi.org/10.1039/d4nr05294c

Golan, N., Parizat, A., Tabachnikov, O., Barnea, E., Olsen, W. P., Otzen, D. E. & Landau, M. (2025). Resilience and Charge-Dependent Fibrillation of functional amyloid: Interactions of Pseudomonas Biofilm-Associated FapB and FapC Amyloids. The Journal of Biological Chemistry, 301(2), Article 108096. https://doi.org/10.1016/j.jbc.2024.108096

Jiang, Y. & Kjems, J. (2025). RNA denaturation underlies circular RNA separation. Nucleic Acids Research, 53(21), Article gkaf1160. https://doi.org/10.1093/nar/gkaf1160

Nowak, J. S., Olesen, S., Tian, P., Bærentsen, R. L., Brodersen, D. E. & Otzen, D. E. (2025). Role of electrostatics in cold adaptation: A comparative study of eury- and stenopsychrophilic triose phosphate isomerase. Biochimica et Biophysica Acta - Proteins and Proteomics, 1873(4), Article 141072. https://doi.org/10.1016/j.bbapap.2025.141072

Kristoffersen, E. L., McRae, E. K. S., Sørensen, N. R., Holliger, P. & Andersen, E. S. (2025). Roles of dimeric intermediates in RNA-catalyzed rolling circle synthesis. Nucleic Acids Research, 53(11), Article gkaf057. https://doi.org/10.1093/nar/gkaf057

Fage-Butler, A., Ledderer, L. & Nielsen, K. H. (Eds.) (2025). Science communication and trust. Palgrave Macmillan. https://doi.org/10.1007/978-981-96-1289-5

Stenberg, K., Korhonen, S., Seemab, U., Dupont, D. M., Kjems, J., Honkakoski, P. & Subrizi, A. (2025). Selection of locally administered ocular tissue-targeting RNA aptamers using in vivo SELEX. European Journal of Pharmaceutical Sciences, 212, Article 107217. https://doi.org/10.1016/j.ejps.2025.107217

Xia, D., Shi, T., Cao, W., Li, B., Wang, D., Liang, C. & Dong, M. (2025). Self-healing hydrogel based on polyvinyl alcohol/dextran with hyperglycemia-triggered cascade enzyme catalytic activity promotes diabetic wound healing. Journal of Colloid and Interface Science, 693, Article 137615. https://doi.org/10.1016/j.jcis.2025.137615

Baindoor, S., Gibriel, H. A. Y., Kool, L., Su, J., Demaegd, K. C., Venø, M. T., Van Den Berg, L. H., Kjems, J., Van Es, M. A. & Prehn, J. H. M. (2025). Serum small non-coding RNA define molecular subtypes in amyotrophic lateral sclerosis. Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration. Advance online publication. https://doi.org/10.1080/21678421.2025.2574690

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Kalinka Hansen