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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Nielsen, K. H. (2002). Tilting at windmills: On Actor-Worlds, Socio-Logics, and Techno-Economic Networks of Wind Power, 1974-1999.

Guldberg, L. B., Finster, K., Jørgensen, N. O. G., Middelboe, M. & Lomstein, B. A. (2002). Utilization of marine sedimentary dissolved organic nitrogen by native anaerobic bacteria. Limnology and Oceanography, 47(6), 1712-1722.

Bot, A. N. M., Ortius-Lechner, D., Finster, K. & Boomsma, J. J. (2002). Variable sensitivity of fungi and bacteria to compounds produced by the metapleural glands of leaf-cutting ants. Insectes soc., 49, 363-370.

Nilsson, J., Askjaer, P. & Kjems, J. (2001). A role for the basic patch and the C-terminus of RanGTP in regulating the dynamic interaction with importin β, CRM1, and RanBP1. J. Mol. Biol., 305, 231-243.

Thomsen, T. R., Finster, K. & Ramsing, N. B. (2001). Biogeochemical and Molecular Signatures of Anaerobic Methane Oxidation in a Marine Sediment. Applied and Environmental Microbiology, 67(4), 1646-1656.

Jagota, A., Lyngsø, R. B., Pedersen, C. N. S., Gascuel, O. (Ed.) & Moret, B. M. E. (Ed.) (2001). Comparing Hidden Markov Model and a Stochastic Context-Free Grammer. In Proceedings of the First International Workshop on Algorithms in Bioinformatics (2149 of Lecture Notes in Computer Science ed., Vol. Volume 2149/2001, pp. 69-94). Springer.

Lyngsø, R. B., Pedersen, C. N. S., Eades, P. (Ed.) & Takaoka, T. (Ed.) (2001). Complexity of Comparing Hidden Markov Models. In Ikke angivet (2223 of Lecture Notes in Computer Science ed., pp. 416-428). Springer.

Brodal, G. S., Fagerberg, R., Pedersen, C. N. S., Eades, P. (Ed.) & Takaoka, T. (Ed.) (2001). Computing the Quartet Distance Between Evolutionary Trees in Time O(n log² n). In Proceedings of Algorithms and Computation : 12th International Symposium, ISAAC 2001 (2223 of Lecture Notes in Computer Science ed., Vol. 2223/2001, pp. 731-742). Springer.

Poulsen, H., Nilsson, J., Damgaard, C. K., Jensen, J. E. & Kjems, J. (2001). CRM1 mediates the export of ADAR1 through a nuclear export signal within the Z-DNA binding domain. Molecular and Cellular Biology, 21, 7862-7871.

Finster, K., Thomsen, T. R. & Ramsing, N. B. (2001). Desulfomusa hansenii gen.nov., sp.nov., a novel marine propionate-degrading, sulfate-reducing bacterium isolated from Zostera marina roots. International Journal of Systematic and Evolutionary Microbiology, 51, 2055-2061.

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