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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Halkjær, S., Iversen, J., Kyhl, L., Chevallier, J., Andreatta, F., Yu, F., Stoot, A., Camilli, L., Bøggild, P., Hornekær, L. & Cassidy, A. M. (2019). Low-temperature synthesis of a graphene-based, corrosion-inhibiting coating on an industrial grade alloy. Corrosion Science, 152, 1-9. https://doi.org/10.1016/j.corsci.2019.01.029

Nielsen, K. H. (2019). Lsd. Weekendavisen, Sektion 4 (Ideer), 10.

Pedersen, J. N., Jiang, Z., Christiansen, G., Lee, J. C., Pedersen, J. S. & Otzen, D. E. (2019). Lysophospholipids induce fibrillation of the repeat domain of Pmel17 through intermediate core-shell structures. Biochimica et Biophysica Acta - Proteins and Proteomics, 1867(5), 519-528. https://doi.org/10.1016/j.bbapap.2018.11.007

Nielsen, K. H. (2019). Månen. Weekendavisen, Sektion 4 (Ideer), 10.

Liu, J., Champer, J., Langmüller, A. M., Liu, C., Chung, J., Reeves, R., Luthra, A., Lee, Y. L., Vaughn, A. H., Clark, A. G. & Messer, P. W. (2019). Maximum likelihood estimation of fitness components in experimental evolution. Genetics, 211(3), 1005-1017. https://doi.org/10.1534/genetics.118.301893

Mohammad-Beigi, H., Hosseini, A., Adeli, M., Ejtehadi, M. R., Christiansen, G., Sahin, C., Tu, Z., Tavakol, M., Dilmaghani-Marand, A., Nabipour, I., Farzadfar, F., Otzen, D. E., Mahmoudi, M. & Hajipour, M. J. (2019). Mechanistic Understanding of the Interactions between Nano-Objects with Different Surface Properties and α-Synuclein. ACS Nano, 13(3), 3243-3256. https://doi.org/10.1021/acsnano.8b08983

Chen, M., Jørgensen, M. L. & Sikkersoq Kimer Nøddesbo, M. (2019). Melt Electrowritten Filter for Capturing Cells. Manuscript submitted for publication. (Patent No. 19194329.9-1120).

Nielsen, K. H. (2019). Meme. Weekendavisen, Sektion 4 (Ideer), 10.

Fang, X., Wang, Y., Wang, Z., Jiang, Z. & Dong, M. (2019). Microorganism assisted synthesized nanoparticles for catalytic applications. Energies, 12(1), Article 190. https://doi.org/10.3390/en12010190

Ramos-Docampo, M., Fernández Medina, M., Taipaleenmäki, E. M., Hovorka, O., Salgueiriño, V. S. & Stadler, B. M. (2019). Microswimmers with Heat Delivery Capacity for 3D Cell Spheroid Penetration. A C S Nano, 13(10), 12192-12205. https://doi.org/10.1021/acsnano.9b06869

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