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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Besenbacher, S., Mailund, T., Westh-Nielsen, L. & Pedersen, C. N. S. (2004). RBT - A Tool for Building Refined Buneman Trees. Bioinformatics, 21(8), 1711-1712.

Damgaard, C. K., Andersen, E. S., Knudsen, B., Gorodkin, J. & Kjems, J. (2004). RNA interactions in the 5' region of the HIV-1 genome. Journal of Molecular Biology, 336(2), 369-79. https://doi.org/10.1016/j.jmb.2003.12.010

Andersen, E. S., Contera, S., Knudsen, B., Damgaard, C. K., Besenbacher, F. & Kjems, J. (2004). Role of the TAR hairpin in dimerization of HIV-1 RNA. Journal of Biological Chemistry, 279, 22243-22249.

Andersen, E. S., Contera, S. A., Knudsen, B., Damgaard, C. K., Besenbacher, F. & Kjems, J. (2004). Role of the trans-activation response element in dimerization of HIV-1 RNA. Journal of Biological Chemistry, 279(21), 22243-22249. https://doi.org/10.1074/jbc.M314326200

Zahler, A. M., Damgaard, C. K., Kjems, J. & Caputi, M. (2004). SC35 and heterogeneous nuclear ribonucleoprotein A/B proteins bind to a juxtaposed exonic splicing enhancer/exonic splicing silencer element to regulate HIV-1 tat exon 2 splicing. Journal of Biological Chemistry, 279(11), 10077-10084. https://doi.org/10.1074/jbc.M312743200

Zahler, A. M., Damgaard, C. K., Kjems, J. & Caputi, M. (2004). SC35 and hnRNPs A/B binding to a juxtaposed ESE/ESS element regulates HIV-1 tat exon 2 splicing. Journal of Biological Chemistry, 279, 10077-10084.

Hannila, M.-L., Jensen, E. B. V., Hobolth, A. & Nyengaard, J. R. (2004). Shape modelling of spatial particles from planar central sections - a case study. Journal of Microscopy, 215(2), 183-190. https://doi.org/10.1111/j.0022-2720.2004.01364.x

Kristensen, D. M. & Andersen, E. S. (2004). Stamceller - kroppens egne schweizerknive. Jyllands-Posten, Indblik.

Nielsen, K. H. & Nielsen, H. (2004). The Rise of Science Journalism in Denmark. In Public Communication of Science and Technology (PCST-8). Scientific Knowledge and Cultural Diversity. Conference Proceedings

Frederiksen, T.-M. & Finster, K. (2004). The transformation of inorganic sulfur compounds and the assimilation of organic and inorganic carbon by the sulfur disproportionating bacterium Desulfocapsa sulfoexigens. Antonie van Leeuwenboek, 85, 141-149.

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