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AUNAB - Aarhus University Network on Artificial Biology

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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Gal, N., Charwat, V., Städler, B. & Reimhult, E. (2019). Poly(ethylene glycol) grafting of nanoparticles prevents uptake by cells and transport through cell barrier layers regardless of shear flow andpParticle size. ACS Biomaterials Science and Engineering, 5(9), 4355-4365. https://doi.org/10.1021/acsbiomaterials.9b00611

Kang, S., He, M., Chen, M., Wang, Z., Sun, Z., Dang, H., Chang, X., Dong, M., Liu, P. & Cui, L. (2019). Polymeric structure optimization of g-C₃N₄ by using confined argon-assisted highly-ionized ammonia plasma for improved photocatalytic activity. Journal of Colloid and Interface Science, 556, 214-223. https://doi.org/10.1016/j.jcis.2019.08.040

De Vrieze, J., Louage, B., Deswarte, K., Zhong, Z., De Coen, R., Van Herck, S., Nuhn, L., Kaas Frich, C., Zelikin, A. N., Lienenklaus, S., Sanders, N. N., Lambrecht, B. N., David, S. A. & De Geest, B. G. (2019). Potent Lymphatic Translocation and Spatial Control Over Innate Immune Activation by Polymer–Lipid Amphiphile Conjugates of Small-Molecule TLR7/8 Agonists. Angewandte Chemie - International Edition, 58(43), 15390-15395. https://doi.org/10.1002/anie.201905687

Nielsen, S. B. & Otzen, D. E. (2019). Quartz Crystal Microbalances as Tools for Probing Protein-Membrane Interactions. In J. H. Kleinschmidt (Ed.), Methods in Molecular Biology: Methods and Protocols (Vol. 2003, pp. 31-52). Springer. https://doi.org/10.1007/978-1-4939-9512-7_2

Westberg, M., Etzerodt, M. & Ogilby, P. R. (2019). Rational Design of Genetically Encoded Singlet Oxygen Photosensitizing Proteins. Current Opinion in Structural Biology, 57(August), 56-62. https://doi.org/10.1016/j.sbi.2019.01.025

Zhu, X., Ding, R., Wang, Z., Wang, Y., Guo, X., Song, Z., Wang, Z. & Dong, M. (2019). Recent advances in synthesis and biosensors of two-dimensional MoS₂. Nanotechnology, 30(50), Article 502004. https://doi.org/10.1088/1361-6528/ab42fe

Qian, X. & Städler, B. (2019). Recent Developments in Polydiacetylene-Based Sensors. Chemistry of Materials, 31(4), 1196-1222. https://doi.org/10.1021/acs.chemmater.8b05185

Christensen, L. F. B., Jensen, K. F., Nielsen, J., Vad, B. S., Christiansen, G. & Otzen, D. E. (2019). Reducing the Amyloidogenicity of Functional Amyloid Protein FapC Increases Its Ability To Inhibit α-Synuclein Fibrillation. ACS Omega, 4(2), 4029-4039. https://doi.org/10.1021/acsomega.8b03590

Bortolini, C., Kartanas, T., Copic, D., Condado Morales, I., Zhang, Y., Challa, P. K., Peter, Q., Jávorfi, T., Hussain, R., Dong, M., Siligardi, G., Knowles, T. P. J. & Charmet, J. (2019). Resolving protein mixtures using microfluidic diffusional sizing combined with synchrotron radiation circular dichroism. Lab on a Chip, 19(1), 50-58. https://doi.org/10.1039/c8lc00757h

Eriksen, C. B. (2019). Rezension zu: Östling, Johan; Sandmo, Erling; Larsson Heidenblad, David; Nilsson Hammar, Anna; Nordberg, Kari H. (Hrsg.): Circulation of Knowledge. Explorations in the History of Knowledge. Lund 2018 , ISBN 978-91-88661-28-9. H-Soz-Kult.

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Kalinka Hansen