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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Jensen, A.-M. L., Sørensen, T. L.-M., Olesen, C., Møller, J. V. & Nissen, P. (2006). Modulatory and catalytic modes of ATP binding by the calcium pump. EMBO Journal, 25(11), 2305-2314.

Grunwald, T., Bockisch, B., Spillner, E., Bredehorst, R., Ring, J. & Ollert, M. W. (2006). Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3). Journal of Allergy and Clinical Immunology, 117(4), 848-854. https://doi.org/10.1016/j.jaci.2005.12.1331

Pedersen, J. S., Dikov, D. & Otzen, D. (2006). N- and C-Terminal Hydrophobic Patches Are Involved in Fibrillation of Glucagon. Biochemistry, 45(48), 14503-14512. https://doi.org/10.1021/bi061228n

Ebbesen, M. & Andersen, S. (2006). Nanoethics: General Principles and Christian Discourse. Journal of Lutheran Ethics, (2).

Ebbesen, M. (2006). Nanofood - Lessons to be Learnt from the Debate on GM Crops? In M. Kayser & M. Lien (Eds.), Ethics and the Politics of Food (pp. 314-320). Wageningen Academic Publishers.

Ebbesen, M. & Jensen, T. G. (2006). Nanomedicine: techniques, potentials, and ethical implications. Journal of Biomedicine and Biotechnology, 2006(5), 51516. https://doi.org/10.1155/JBB/2006/51516

Ebbesen, M. & Jensen, T. G. (2006). Nanomedicine: Techniques, Potentials, and Ethical Implications. Journal of Biomedicine and Biotechnology, 2006, 1-11.

Kjems, J., Liu, X., Howard, K. & Besenbacher, F. (2006). Nanoparticles for nuclear acid delivery.

Nielsen, H. & Nielsen, K. H. (2006). Naturvidenskabens store spring fremad. Aktuel Naturvidenskab, (4), 28-31.

Kjærgaard, P. C., Kragh, H., Nielsen, K. H. & Nielsen, H. (2006). Naturvidenskabernes Danmarkshistorie. Magisterbladet, (10).

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