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Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Andersen, F. F., Tange, T. Ø., Sinnathamby, T., Lohmann, J. S., Andersen, K. E., Kjems, J., Westergaard, O. & Knudsen, B. R. (2003). The RNA splicing factor ASF/SF2 inhibits human topoisomerase I mediated DNA relaxation. Poster session presented at Mechanisms of Eukaryotic Transcription, Cold Spring Harbor, United States.

Sand-Jensen, K., Kjaer, C. R., Dahlgaard, J. & Andersen, E. S. (2003). Ud af osteklokken. Aktuel Naturvidenskab, (4), 28-29.

Lindtner, S., Felber, B. K. & Kjems, J. (2002). An element in the 3′untranslated region of human LINE-1 retrotransposon mRNA binds NXT1 (TAP) and can function as a nuclear export element. RNA, 8, 345-356.

Hobolth, A. & Jensen, E. B. V. (2002). A note on design-based versus model-based variance estimation in stereology. Advances in Applied Probability, 34, 484-490. https://doi.org/10.1239/aap/1033662161

Daelemans, D., Afonina, E., Nilsson, J., Werner, G., Kjems, J., Clercq, E., Pavlakis, G. N. & Vandamme, A. (2002). A novel inhibitor of the CRM-1 mediated pathway. Proceedings of the National Academy of Sciences (PNAS), 99, 14440-14445.

Hansen, A., Nørberg, P., Merrison, J., Lomstein, B. A. & Finster, K. (2002). A simulation facility for biological experiments. Paper presented at Graz, Austria, 16-19 September (ESA SP-518, November 2002).

Pedersen, C. N. S. (2002). Bioinformatik ved Aarhus Universitet. Biozoom, (3), 12-18.

Pedersen, C. N. S., Scharling, T., Guigó, R. (Ed.) & Gusfield, D. (Ed.) (2002). Comparative Methods for Gene Structure Prediction in Homologous Sequences. In Ikke angivet (2452 of Lecture Notes in Computer Science ed., Vol. 2452/2002, pp. 220-234). Springer.

Lund, M. & Kjems, J. (2002). Defining a 5′ splice site by functional selection in the presence and absence of U1 snRNA 5′-end. RNA, 8, 166-179.

Finster, K., Jensen, A.-M., Hansen, A., Lomstein, B. A., Nørberg, P. & Merrison, J. P. (2002). Description of a Mars-simulation facility. Paper presented at Graz, Austria, 16-19 September 2002.

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