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Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Christensen, L. F. B., Nowak, J. S., Sønderby, T. V., Frank, S. A. & Otzen, D. E. (2020). Quantitating denaturation by formic acid: Imperfect repeats are essential to the stability of the functional amyloid protein FapC. Journal of Biological Chemistry, 295(37), 13036-13041. https://doi.org/10.1074/jbc.ra120.013396

Taipaleenmäki, E. & Städler, B. (2020). Recent Advancements in Using Polymers for Intestinal Mucoadhesion and Mucopenetration. Macromolecular Bioscience, 20(3), Article 1900342. https://doi.org/10.1002/mabi.201900342

Fernández-Medina, M., Ramos-Docampo, M. A., Hovorka, O., Salgueiriño, V. & Städler, B. (2020). Recent Advances in Nano- and Micromotors. Advanced Functional Materials, 30(12), Article 1908283. https://doi.org/10.1002/adfm.201908283

Valero, J. & Famulok, M. (2020). Regeneration of Burnt Bridges on a DNA Catenane Walker. Angewandte Chemie - International Edition, 59(38), 16366-16370. https://doi.org/10.1002/anie.202004447

Kania, K., Colella, F., Riemen, A. H. K., Wang, H., Howard, K., Aigner, T., Dell’Accio, F., Capellini, T. D., Roelofs, A. J. & De Bari, C. (2020). Regulation of Gdf5 expression in joint remodelling, repair and osteoarthritis. Scientific Reports, 10(1), 157. Article 157. https://doi.org/10.1038/s41598-019-57011-8

Godskesen, L. E., Lassen, T. R., Jespersen, N. R., Siersbæk, M., Yan, Y., Nielsen, M. M., Tjønnfjord, S. K., Grøntved, L., Madsen, G., Kjems, J., Bøtker, H. E., Schmidt, M. R., Krag, A. & Kjeldsen, J. (2020). Remote ischemic conditioning in active ulcerative colitis: An explorative randomized clinical trial. Scientific Reports, 10(1), Article 9537. https://doi.org/10.1038/s41598-020-65692-9

Pedersen, S. L., Huynh, T. H., Pöschko, P., Fruergaard, A. S., Jarlstad Olesen, M. T., Chen, Y., Birkedal, H., Subbiahdoss, G., Reimhult, E., Thøgersen, J. & Zelikin, A. N. (2020). Remotely Triggered Liquefaction of Hydrogel Materials. ACS Nano, 14(7), 9145-9155. https://doi.org/10.1021/acsnano.0c04522

Bailey, M. H., Meyerson, W. U., Dursi, L. J., Wang, L. B., Dong, G., Liang, W. W., Weerasinghe, A., Li, S., Kelso, S., Akbani, R., Anur, P., Bailey, M. H., Buchanan, A., Chiotti, K., Covington, K., Creason, A., Ding, L., MC3 Working Group, PCAWG novel somatic mutation calling methods working group & PCAWG Consortium (2020). Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples. Nature Communications, 11(1), Article 4748. https://doi.org/10.1038/s41467-020-18151-y

Wang, Z., Wu, H. H., Li, Q., Besenbacher, F., Li, Y., Zeng, X. C. & Dong, M. (2020). Reversing Interfacial Catalysis of Ambipolar WSe₂ Single Crystal. Advanced Science, 7(3), Article 1901382. https://doi.org/10.1002/advs.201901382

Schiølin, K. (2020). Revolutionary dreams: Future essentialism and the sociotechnical imaginary of the fourth industrial revolution in Denmark. Social Studies of Science, 50(4), 542-566. https://doi.org/10.1177/0306312719867768

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