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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

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Cai, Y., López-Ruiz , E., Wengel, J., Creemers, L. & Howard, K. (2017). A hyaluronic acid-based hydrogel enabling CD44-mediated chondrocyte binding and gapmer oligonucleotide release for modulation of gene expression in osteoarthritis. Journal of Controlled Release, 253(May), 153-159. https://doi.org/10.1016/j.jconrel.2017.03.004

Caspersen , M. B., Kuhlmann, M., Nicholls , K., Saxton , M. J., Andersen , B., Bunting , K., Cameron, J. & Howard, K. (2017). Albumin-based drug delivery using cysteine 34 chemical conjugates; important considerations and requirements. Therapeutic Delivery, 8(7), 511-519. https://doi.org/10.4155/tde-2017-0038

Nielsen, K. H. (2017). Algoritme. Weekendavisen, Sektion 4 (Ideer), 10.

Nielsen, K. H. (2017). Allergi. Weekendavisen, Sektion 4 (Ideer), 10.

Jensen-Jarolim, E., Bax, HJ., Bianchini, R., Capron, M., Corrigan, C., Castells, M., Dombrovicz, D., Daniels-Wells, HR., Fazekas, J., Fiebiger, E., Gatault, S., Gould, H., Janda, J., Josephs, DH., Karagiannis, P., Levi-Schaffer, F., Meshcheryakova, A., Mechtcheriakova, D., Mekori, Y. ... Karagiannis, S. N. (2017). AllergoOncology - the Impact of Allergy in Oncology. EAACI Position Paper. Allergy: European Journal of Allergy and Clinical Immunology, 72(6), 866-887. https://doi.org/10.1111/all.13119

Nielsen, K. H. (2017, Feb 5). Alt det, vi ved, vi ikke ved - og alt det, vi ikke ved, vi ikke ved. Videnskab.dk. https://videnskab.dk/kultur-samfund/der-er-det-vi-ved-vi-ikke-ved-og-saa-er-der-alt-det-vi-ikke-ved-vi-ikke-ved

Rajman, M., Metge, F., Fiore, R., Khudayberdiev, S., Aksoy-Aksel, A., Bicker, S., Reschke, C. R., Raoof, R., Brennan, G. P., Delanty, N., Farrell, M. A., O'Brien, D. F., Bauer, S., Norwood, B., Veno, M. T., Krueger, M., Braun, T., Kjems, J., Rosenow, F. ... Schratt, G. (2017). A microRNA-129-5p/Rbfox crosstalk coordinates homeostatic downscaling of excitatory synapses. EMBO Journal, 36(12), 1770-1787. https://doi.org/10.15252/embj.201695748

Uhd, J., Okholm, A., Kjems, J. & Astakhova, K. (2017). Amplification-free liquid biopsy by fluorescence approach. In Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017 (Vol. 3, pp. 106-109). TechConnect. https://briefs.techconnect.org/wp-content/volumes/TCB2017v3/pdf/762.pdf

Kuhlmann, M., Hamming, J. B. R., Voldum, A., Tsakiridou, G., Larsen, M. T., Schmøkel, J., Sohn, E., Bienk, K., Schaffert, D. H., Sørensen, E. S., Wengel, J., Dupont, D. M. & Howard, K. (2017). An albumin-oligonucleotide assembly for potential combinatorial drug delivery and half-life extension applications. Molecular Therapy - Nucleic Acids, 9, 284-293. https://doi.org/10.1016/j.omtn.2017.10.004

Schrempf, D. & Hobolth, A. (2017). An alternative derivation of the stationary distribution of the multivariate neutral Wright–Fisher model for low mutation rates with a view to mutation rate estimation from site frequency data. Theoretical Population Biology, 114, 88-94. https://doi.org/10.1016/j.tpb.2016.12.001

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