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Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

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Halkoaho, A., Pietila, AM., Ebbesen, M., Karki, S. & Kangasnniemi, M. (2015). Cultural aspects related to informed consent in health care research: A systematic review. Nursing Ethics, 1-14. https://doi.org/10.1177/0969733015579312

Zhang, P., Hou, X., Li, S., Liu, D. & Dong, MD. (2015). Curvature effect of O₂ adsorption and dissociation on SiC nanotubes and nanosheet. Chemical Physics Letters, 619, 92-96. https://doi.org/10.1016/j.cplett.2014.11.057

Xiang, F., Lu, Y., Li, C., Song, X., Liu, X., Wang, Z., Liu, J. & Dong, M. (2015). Cyclotrimerization-Induced Chiral Supramolecular Structures of 4-Ethynyltriphenylamine on Au(111) Surface. Chemistry: A European Journal, 21(37), 12978-12983. https://doi.org/10.1002/chem.201501434

Nielsen, K. H. (2015). Danske forskere trues til tavshed. Politiken, Debat, 6. http://politiken.dk/debat/ECE2868635/danske-forskere-trues-til-tavshed/

Möbs, C., Müller, J., Rudzio, A., Pickert, J., Blank, S., Jakob, T., Spillner, E. & Pfützner, W. (2015). Decline of Ves v 5-specific blocking capacity in wasp venom-allergic patients after stopping allergen immunotherapy. Allergy: European Journal of Allergy and Clinical Immunology, 6. https://doi.org/10.1111/all.12606

Li, Y., Femenias, M. R., Yu, Y., Besenbacher, F. & Chen, M. (2015). Delivery of Dexamethasone from electrospun PCL-PEO binary fibers and their effects on inflammation regulation. R S C Advances, 5, 34166-34172. https://doi.org/10.1039/c5ra03099d

Nielsen, K. H. (2015). Den personlige computer. In H. Kragh & K. H. Nielsen (Eds.), 50 opfindelser: Højdepunkter i teknologien (pp. 305-309). Aarhus Universitetsforlag.

Xue, Y., Wang, L., Xia, D., Li, Q., Gao, S., Dong, MD., Cai, T., Shi, S., He, L., Hu, K., Mao, T. & Duan, X. (2015). Dental Abnormalities Caused by Novel Compound Heterozygous CTSK Mutations. Journal of Dental Research, 94(5), 674-681. https://doi.org/10.1177/0022034515573964

Pijanka, J. K., Spang, M. T., Sorensen, T., Liu, J., Nguyen, T. D., Quigley, H. A. & Boote, C. (2015). Depth-dependent changes in collagen organization in the human peripapillary sclera. PLoS One, 10(2), e0118648. https://doi.org/10.1371/journal.pone.0118648

Spillner, E. (2015). Diagnostik der Insektengiftallergie: vom Extrakt zum Molekül. Spitzenforschung in der Allergologie, 2015, 92-93.

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