Publications

Recent research news on Artificial Biology

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer-Lipid Hybrid Vesicles

Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a major goal in bottom-up synthetic biology. In this study, we demonstrate the in situ polymerization of microtubules encapsulated in giant polymer-lipid hybrid vesicles (GHVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine and an amphiphilic block copolymer. The block copolymer is comprised of poly(cholesteryl methacrylate-co-butyl methacrylate) as the hydrophobic block and either poly(6-O-methacryloyl-D-galactopyranose) or poly(carboxyethyl acrylate) as the hydrophilic extension. Depending on the concentrations of guanosine triphosphate (GTP) or its slowly hydrolyzable analog, guanosine-5′-[(α,β)-methyleno]triphosphate (GMPCPP), different microtubule morphologies are observed, including encapsulated microtubule networks, spike protrusions, as well as membrane-associated or aggregated microtubules. Overall, this work represents a step forward in mimicking the cellular cytoskeletons and uncovering the influence of membrane composition on microtubule morphologies.

Recent publications by network

Sort by: Date | Author | Title

Andersen, E. S. (2002). Kapitler i livets bog. Information.

Nielsen, K. H. (2002). M. J. Pasqualetti, P. Gipe, and R. W. Righter. Wind Power in View. Energy Landscapes in a Crowded World. Technology and Culture, 43(4), 825-826.

Andersen, E. S. (2002). Niles Eldredge - palæontologi og evolutionsteori. Pioneers in Biology 2002, 10-11.

Revsbech, N. P. & Finster, K. (2002). Oxygen: Effect on marine microbial communities. In Encyclopedia of Microbial Ecology (pp. 2277-2288). Wiley, New York.

Hein, J., Jensen, J. L. & Pedersen, C. N. S. (2002). Recursions for Statistical Multiple Alignment. Department of Mathematical Sciences , University of Aarhus.

Hein, J., Pedersen, C. N. S. & Jensen, J. L. (2002). Recursions for Statistical Multiple Alignment. Proceedings of the National Academy of Sciences (PNAS), 100(25), 14960-14965.

Dirac, A. M., Huthoff, H., Kjems, J. & Berkhout, B. (2002). Regulated HIV-2 RNA dimerization by means of alternative RNA conformation. Nucleic Acids Research, 30, 2647-2655.

A.M., D., H., H., Kjems, J. & B., B. (2002). Requirements for RNA-heterodimerization of the HIV-1 and HIV-2 genomes. Journal of General Virology, 83, 2533-2542.

Spillner, E. (2002). Selektion und Expression von rekombinanten Antikörpern für analytische und therapeutische Applikationen. Cuvillier Verlag.

Nørnberg, P., Merrison, J. P., Finster, K., Folkmann, F., Gunnlaugsson, H. P., Hansen, A., Jensen, A. M., Kinch, K., Lomstein, B. A. & Mugford, R. (2002). Simulation of martian surface conditions and dust transport. Paper presented at Graz, Austria, 16-19 September (ESA SP-518,November 2002).

Displaying results 3381 to 3390 out of 3469

339